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We narrowed to 18,031 results for: Mut

Showing: 3261 - 3280 of 18031 results

  9. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  10. pIS1 mEPAS1

    Plasmid
    #60793
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    EPAS1 3'UTR and mutated miR-155 binding site (EPAS1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60793-plasmid-map-sequence-id-112560

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  11. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid
    #172597
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-K627R (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R
    Promoter
    CMV
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172597-plasmid-map-sequence-id-338908

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  12. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available Since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

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  13. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid
    #122064
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1a（1.1kb short version)
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122064-plasmid-map-sequence-id-242270

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  14. xPERT-hA2q

    Plasmid
    #231063
    Purpose
    Circularly permuted CasRx platform fused to human deaminase domain for A to I editing
    Depositor
    Meng How Tan
    Article
    Wang et al Nat Biotechnol. 2024 Oct 9. doi: 10.1038
    Inserts
    dCas13d
    Human deaminase ADAR2dd (ADARB1 Ruminococcus flavefaciens XPD3002)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H460D, E488Q, only the deaminase domain (aa 276-7…
    Promoter
    CMV
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231063-plasmid-map-sequence-id-459741

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  15. AAV-KrasG12D/sgKras/Cre

    Plasmid
    #99851
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99851-plasmid-map-sequence-id-193760

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  17. AAV-KrasG12C/sgKras/Cre

    Plasmid
    #99850
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99850-plasmid-map-sequence-id-193772

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  18. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid
    #84483
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84483-plasmid-map-sequence-id-177044

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  20. AAV-KrasG12V/sgKras/Cre

    Plasmid
    #99854
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99854-plasmid-map-sequence-id-193790

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Showing: 3261 - 3280 of 18031 results