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pDisplay-GRAB_CRF1.0

Plasmid

#208655

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Expression

Mammalian

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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ELK1 pcDNA3.1/HisC

Plasmid

#121093

Purpose

Expression of full length ELK1 in mammalian cells

Depositor

Paul Herring

Publication

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

6XHis-Omni-xpress

Expression

Mammalian

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCYP119

Plasmid

#50771

Purpose

expresses CYP 119 in e. coli

Depositor

Stephen Sligar

Publication

McLean et al Biochem Biophys Res Commun. 1998 Nov 9;2

Insert

CYP119

Expression

Bacterial

Promoter

lac

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-HsSmg6_H

Plasmid

#146475

Purpose

Mammalian Expression of HsSmg6

Depositor

Elisa Izaurralde

Publication

Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

Insert

HsSmg6 (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD63-(TEV)-tTA

Plasmid

#162597

Purpose

human CD63-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Publication

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD63 (CD63 Human)

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HOXB1-pSG5

Plasmid

#20981

Depositor

Corey Largman

Publication

Mathews et al Blood. 1991 Nov 1. 78(9):2248-52.

Insert

HOXB1 (HOXB1 Human)

Expression

Mammalian

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pAAV_SynP_DIO_tAKARα- T391A_WPRE

Plasmid

#196676

Purpose

AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

Depositor

Haining Zhong

Publication

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα-T391A

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSSmGFP

Plasmid

#186534

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A

Plasmid

#172201

Purpose

Expresses KIF5A tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Publication

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HN

Plasmid

#104171

Purpose

Cas9-WT mammalian expression

Depositor

Jin-Soo Kim

Publication

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-WT

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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NavBp

Plasmid

#100014

Purpose

Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

Depositor

David Clapham, Paul DeCaen

Publication

DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

Insert

NavBp

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pNeoXTR f2

Plasmid

#69159

Purpose

mouse targeting vector

Depositor

David Feldser

Publication

Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

Inserts

DTA
NeoR
GFP
AmpR

Use

Mouse Targeting

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3xFNLDD/pMXs-puro

Plasmid

#49536

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Publication

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

LTR

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP

Use

Lentiviral

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only