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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,362 results for: Coli

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Showing: 6561 - 6600 of 10362 results

  1. pFLIP37

    Plasmid
    #65505
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-Cit-attR1 and attR2-Cer-cMyc
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65505-plasmid-map-sequence-id-111225

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  14. pRL1058

    Plasmid
    #70686
    Purpose
    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.
    Depositor
    Peter Wolk
    Article
    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88
    Insert
    Transposon Tn5-1058
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70686-plasmid-map-sequence-id-126440

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  20. pFLIP30

    Plasmid
    #65499
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-AFPt9-attR1 and attR2-mCer-cMyc
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65499-plasmid-map-sequence-id-111219

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  21. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid
    #73221
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL31-sgRNA NT1
    Expression
    Bacterial
    Promoter
    PL31
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73221-plasmid-map-sequence-id-135054

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  23. pFLIP32

    Plasmid
    #65500
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65500-plasmid-map-sequence-id-111220

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  24. pFLIP39

    Plasmid
    #65507
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65507-plasmid-map-sequence-id-111416

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  33. pGRNA3_LacZ_IB1_IIT1

    Plasmid
    #247080
    Purpose
    L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and T1 site. riboJ/RNaseIII site are encoded between two crRNA
    Depositor
    Seokhee Kim
    Article
    Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4
    Insert
    type I-E CRISPR cas crRNA
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247080-plasmid-map-sequence-id-491602

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  39. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222017
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    deleted stop codon (TAA)
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222017-plasmid-map-sequence-id-457152

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Showing: 6561 - 6600 of 10362 results