We narrowed to 6,241 results for: nls

1. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Flag-HFSpCas9-H840A

   Plasmid

   #80455

   Purpose

   high fidelity SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   HFSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-RfxCas13d-Blast-BFP

   Plasmid

   #196726

   Purpose

   Sortable and selectable RfxCas13d.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-ABE8e-NRCH

   Plasmid

   #165416

   Purpose

   Expresses the base editor ABE8e-NRCH in mammalian cells

   Depositor

   David Liu

   Insert

   ABE8e-NRCH
   

   Tags

   BP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

   Plasmid

   #79785

   Purpose

   genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

   Depositor

   Anthony Zador

   Insert

   MAPP-nL
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Expression

   Mammalian

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRha-ABE8e-NRCH

   Plasmid

   #165417

   Purpose

   For bacterial purification of ABE8e-NRCH protein

   Depositor

   David Liu

   Insert

   ABE8e-NRCH
   

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

   Plasmid

   #129111

   Purpose

   Homologous recombination vector for excision reporter 1 in the CLYBL locus.

   Depositor

   Bruce Conklin

   Insert

   LSL, eGFP, NLS-RFP
   

   Use

   Cre/Lox and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-HeFSpCas9-H840A

   Plasmid

   #80456

   Purpose

   high fidelity enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   HeFSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEvol-pAcFRS.2.t1

   Plasmid

   #73544

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.2.t1
   pAcFRS.2.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

    Plasmid

    #188768

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Expression

    Bacterial

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Liang Zhang, Jian Yan

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. DNMT3A-dCas9

    Plasmid

    #100090

    Purpose

    DNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-DNMT3A-dCas9-NLS

    Expression

    Mammalian

    Mutation

    includes aa 602-912

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CARPID dCasRx-BASU

    Plasmid

    #153303

    Purpose

    express CARPID dCasRx-BASU fusion protein in mammalian cells

    Depositor

    Liang Zhang, Jian Yan

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    dCasRx-BASU
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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