We narrowed to 6,592 results for: nls
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Plasmid#132642PurposeUsed for in vitro transcription of dual NLS ErCas12a mRNADepositorInsertErCas12a
UseCRISPR; In vitro transcriptionTagsSV40 NLSPromoterT3Available SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9
Plasmid#84243PurposeExpresses ABA-inducible VPR-Sa dCas9DepositorInsertsABI-tagBFP-Sa dCas9
PYL1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable SinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNCX myr Akt1
Plasmid#17245DepositorInsertAkt1 (Akt1 Mouse)
UseRetroviralTagsMyrExpressionMammalianMutationConstitutively activeAvailable SinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_WT
Plasmid#98657PurposeExpresses 6xHis-tagged hnRNPA2 AA 190-341DepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-K20A/R21A (pJUL1774)
Plasmid#131312PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).DepositorInsertbpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-YE1-BE4max
Plasmid#198549PurposeExpresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENExpressionMammalianAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TC1316
Plasmid#164883PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3DepositorInsertdcas13d-ADARdd
ExpressionMammalianMutationdCas13d L560-G586 deletedPromoterCMV, T7Available SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-E2
Plasmid#194590PurposeBacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertE2 (S )
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8.17-m+V106W
Plasmid#198534PurposeExpresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-F1
Plasmid#194592PurposeBacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1 (S )
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1
Plasmid#60540PurposeDonor vector for human MLK1DepositorInsertMLK1 (MAP3K9 Human)
UseGateway CloningAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST1374-scFv-APOBEC-UGI-GB1
Plasmid#113029PurposeExpresses scFv-rAPOBEC1-UGI in mammalian cellsDepositorInsertscFv-rAPOBEC1-UGI-GB1
ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP64 (B1016)
Plasmid#92037PurposeExpresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)DepositorInsertCIB1-VP64 (CIB1 Mustard Weed)
TagsFusion of plant CIB1 with VP64 activation domain.…ExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA
Plasmid#89874Purposeexpresses DRD2-iTango component in mammalian cells for AAV productionDepositorInsertDRD2-V2tail-TevN-BLITz1-TetR-VP16
UseAAVTagsFLAGExpressionMammalianMutationshort isoform of DRD2 (deletion of 242-270)PromoterHuman synapsinAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
Plasmid#218206PurposeZebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert+15.2prox1a
UseZebrafish expressionAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FGFR4
Plasmid#60531PurposeEntry vector for human FGFR4DepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTRW22K_7Ti1
Plasmid#177293Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZDepositorInsertlacZ
UseSynthetic Biology; Yeast expression, rrn genomic …ExpressionBacterialAvailable SinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-V82G (pJUL1828)
Plasmid#131313PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).DepositorInsertbpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2019AvailabilityAcademic Institutions and Nonprofits only