We narrowed to 6,582 results for: cre encoding plasmid

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pGP-AAV-CAG-flex-iGABASnFR2(no bind)-WPRE

Plasmid

#218882

Purpose

AAV-mediated expression of improved GABA sensor control (no change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2(no bind)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F102G R168P

Promoter

CAG

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA (AAV5)

Viral Prep

#230681-AAV5

Purpose

Ready-to-use AAV5 particles produced from AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA (#230681). In addition to the viral particles, you will also receive purified AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA plasmid DNA. minBG-driven expression of iCre recombinase in specific populations of brain cells. These AAV preparations are suitable purity for injection into animals.

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Promoter

minBG

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

Plasmid

#64221

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

Plasmid

#64220

Purpose

Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

Expression

Mammalian

Promoter

CBh and U6

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

Plasmid

#202608

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-2Psub-Kv-WPRE

Plasmid

#247323

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2Psub in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Carolan et al Nat Commun. 2025 Oct 3;16(1):8834. doi:

Insert

JEDI-2Psub-Kv

Use

AAV and Cre/Lox

Tags

Kv

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pDHJS4 AS+

Plasmid

#78242

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS-

Plasmid

#78245

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 BS+

Plasmid

#78244

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AS-

Plasmid

#78243

Purpose

One of four plasmids needed to create the quadruple Holliday junction substrate (QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AN-

Plasmid

#78247

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pDHJS4 AN+

Plasmid

#78246

Purpose

One of four plasmids needed to create the mismatched quadruple Holliday junction substrate (MM-QHJS).

Depositor

Tao-shih Hsieh

Article

Chen et al Nucleic Acids Res. 2013 Mar 1;41(5):e60.

Inserts

attB
attP

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS-SARS2-S-D614 (C-flag)

Plasmid

#156420

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pOGS539_haPD-1

Plasmid

#226716

Purpose

Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

High affinity PD-1 microbody

Tags

HA tag and Mating factor alpha secretion signal

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanCOL2A1enhancer

Plasmid

#173961

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region from COL2A1 gene

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

Plasmid

#169188

Purpose

To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

Plasmid

#169187

Purpose

To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp8 (ORF1ab SARS-CoV-2, Synthetic)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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