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Flag_HsIRE1a_deltaP29_D408_pBabePuro

Plasmid

#58422

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids P29 to D408

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/8R]

Plasmid

#177572

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-VIP [L108/92R]

Plasmid

#177453

Purpose

Mammalian Expression Plasmid of anti-VIP (Human). Derived from hybridoma L108/92.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VIP (Homo sapiens) recombinant mouse monoclonal antibody (VIP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/15R]

Plasmid

#225376

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat) IgG2a R-mAb. Derived from hybridoma N110/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neuroligin-3 (Rattus norvegicus) recombinant (Mouse) monoclonal antibody. (Nlgn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TARPGamma2/4/8 [N245/36R]

Plasmid

#177522

Purpose

Mammalian Expression Plasmid of anti-TARPGamma2/4/8 (Rat). Derived from hybridoma N245/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TARPGamma2/4/8 (Rattus norvegicus) recombinant mouse monoclonal antibody (Tarp Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N425/45]

Plasmid

#190326

Purpose

Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

Plasmid

#210767

Purpose

Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

DRSR 514-517 to AS; 557 R to A; multiple silent m…

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-S-tag [N56/9R]

Plasmid

#206600

Purpose

Mammalian Expression Plasmid of anti-S-tag (Bovine). Derived from hybridoma N56/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-S-tag (Bos taurus) recombinant Mouse monoclonal antibody (RNASE1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VDAC1 [N152B/23R-2b]

Plasmid

#222171

Purpose

Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody. (VDAC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/34R]

Plasmid

#220419

Purpose

Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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VGlut1 scFv [N28/9]

Plasmid

#182084

Purpose

Mammalian Expression of VGlut1 scFv. Derived from hybridoma N28/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting VGlut1 (Rattus norvegicus) (Slc17a7 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Neuroligin-2 scFv [L107/95]

Plasmid

#206764

Purpose

Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/95 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mCherry-Rab8A

Plasmid

#194333

Purpose

For use in light-induced Rab8a protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab8a (RAB8A Canis Lupus)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-B KD/MD

Plasmid

#226410

Purpose

Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-B (VAPB Human)

Tags

HA, T7-Xpress tag, and mCherry

Expression

Mammalian

Mutation

K87D/M89D mutant, unable to bind FFAT motifs

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Pan-QKI scFv [N147/6]

Plasmid

#211605

Purpose

Mammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Aldh1L1 scFv [N103/31]

Plasmid

#190522

Purpose

Mammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Aldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Synapsin [L125/129R]

Plasmid

#177472

Purpose

Mammalian Expression Plasmid of anti-Pan-Synapsin (Human). Derived from hybridoma L125/129.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Synapsin (Homo sapiens) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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