We narrowed to 7,140 results for: 1-Oct
-
Plasmid#49463PurposeHA-tagged HIF-1a Ser668 resuide mutated to AlaDepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHAExpressionMammalianMutationSerine 668 to AlaninePromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-dHeFSpCas9-VP64-6xHis
Plasmid#92119PurposeExpression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive HeFSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…PromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D301-380
Plasmid#19852DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 301 to 380PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA
Plasmid#108837PurposeInsert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA
Plasmid#108840PurposeInsert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA
Plasmid#108841PurposeInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA
Plasmid#108847PurposeInsert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA
Plasmid#108836PurposeInsert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA
Plasmid#108842PurposeInsert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATF4 17: 5’ATF4.uORF1stopm1&2.GFP
Plasmid#21863DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStop codons between the colinear uORF1 and uORF2 …PromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66-2xStrep
Plasmid#130953PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length)PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBC2-PuroIII
Plasmid#153420PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 T450A
Plasmid#19843DepositorInsertMKL1 T450A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Threonine 450 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX330_ATP1A1_G3_Dual_sgRNA
Plasmid#173205PurposeCoselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.DepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
UseCRISPRTagsExpressionMammalianMutationPromoterDual U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-MKL1 D381-506
Plasmid#19853DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 381 to 506PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA
Plasmid#108838PurposeInsert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYL,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA
Plasmid#108839PurposeInsert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA
Plasmid#108834PurposeInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBP,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Plasmid#108835PurposeInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -