We narrowed to 5,403 results for: puc

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-miniTurbo

Plasmid

#207692

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCalpha EV (PKA catalytic subunit Calpha)

Plasmid

#15310

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACA (Prkaca Mouse)

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
qTAG-N-Blast-miniTurbo

Plasmid

#207681

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-69: NPM1-mTagRFP-T

Plasmid

#124608

Purpose

Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1

Depositor

Allen Institute for Cell Science

Insert

NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

May 13, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pSEED-mGreenLantern

Plasmid

#219643

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Enhanced Repressor Domain for TAL Effector (SID4X)

Plasmid

#43882

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

SID4X Domain

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
qTAG-C-sTagRFP-Puro

Plasmid

#207723

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199482

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199483

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-moxGFP

Plasmid

#207677

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199481

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Zeo

Plasmid

#207735

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-miRFP670nano3

Plasmid

#207702

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSEED-mCherry

Plasmid

#219657

Purpose

Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTn7xTS-dTomato

Plasmid

#117391

Purpose

Tn7 tagging vector pTn7xTS with dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TC1405

Plasmid

#164877

Purpose

BFP-preSTOP-GFP reporter

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

BFP-preSTOP-GFP

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7005

Plasmid

#136944

Purpose

Malachite green aptamer with stability hairpin, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

Malachite green aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSK-Sasg

Plasmid

#214350

Purpose

Expresses cloning backbone for SlugCas9 sgRNA

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 tracrRNA

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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