We narrowed to 73,573 results for: LAS

1. pdCas9-humanized

   Plasmid

   #44246

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dead Cas9 with 3X NLS
   

   Use

   CRISPR and Retroviral; Vector

   Tags

   3x NLS

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

   Plasmid

   #219548

   Purpose

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The (SAGG)x4 linker between GAL4(1-65) and the PC…

   Promoter

   CMVd1

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMLS-SV40-EGFP

   Plasmid

   #46919

   Purpose

   Target EGFP gene that is stably integrated into HEK293 genome

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   GFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmCherry-ATG5

   Plasmid

   #13095

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5 (Atg5 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP/golgin-160

   Plasmid

   #180630

   Purpose

   Expression of WT golgin-160

   Depositor

   Carolyn Machamer

   Article

   Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

   Insert

   golgin-160 (GOLGA3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX304-VHL-IRES-GFP

   Plasmid

   #187641

   Purpose

   VHL Expression vector with GFP

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   VHL (VHL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hWIP1 FLAG

   Plasmid

   #28105

   Depositor

   Lawrence Donehower

   Article

   Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

   Insert

   WIP1 (PPM1D Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLIK-Zeo

   Plasmid

   #25736

   Purpose

   Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-PGK-ABI-dCas9-P2A-mcherry

   Plasmid

   #121513

   Purpose

   expression of AB1-fused-dcas9 plasmids

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   AB1 and P2A-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FKBP-epsin(144-575)-GFP

    Plasmid

    #100733

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    epn1 (Epn1 Mouse)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    clathrin binding middle part aa 144-575

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CD8-mCherry-FRB

    Plasmid

    #100738

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and mCherry

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV126

    Plasmid

    #63192

    Purpose

    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCherry-ATG5-K130R

    Plasmid

    #13096

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5-K130R (Atg5 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K130R

    Promoter

    Available since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG_EGFP-GFE3

    Plasmid

    #79869

    Purpose

    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Beta-actin

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-mEGFP-PKCa-C1

    Plasmid

    #112264

    Purpose

    ITRACKalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase C alpha (PRKCA Bovine)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCherry-CIBN-CreC

    Plasmid

    #26889

    Purpose

    Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN-CreC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-170 of CIB1, aa 106-343 of Cre

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-OSF-BECN1

    Plasmid

    #99328

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    Beclin 1 (BECN1 Human)
    

    Use

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pC4-RhE-FRB-Fis1

    Plasmid

    #68056

    Purpose

    express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

    Insert

    Fis1 (FIS1 Human)
    

    Use

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    Fis1 tail (aa 93-152)

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-BE4-PpAPOBEC1 R33A

    Plasmid

    #138345

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1 R33A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 R33A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of Arg 33 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge