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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 5,633 results for: chia

Showing: 3341 - 3360 of 5633 results

  1. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

    Plasmid
    #92225
    Purpose
    Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue Y35 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92225-plasmid-map-sequence-id-197052

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  2. pETM6-3A2-mCherry

    Plasmid
    #73425
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3A2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3A2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73425-plasmid-map-sequence-id-135325

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  3. pETM6-5F5-mCherry

    Plasmid
    #73422
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 5F5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P5F5 (orthogonal T7-lac variant)
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73422-plasmid-map-sequence-id-135322

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  5. pEcNucC

    Plasmid
    #214050
    Purpose
    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    EcNucC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    lacUV5 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214050-plasmid-map-sequence-id-428997

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  17. pT22Ubm4Eng2bKRPC(#493)

    Plasmid
    #184091
    Purpose
    cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:
    Insert
    4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    4xMyc
    Expression
    Mutation
    ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184091-plasmid-map-sequence-id-363977

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  19. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

    Plasmid
    #155134
    Purpose
    Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b
    Depositor
    Sinisa Urban
    Article
    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910
    Insert
    GlpG (glpG Escherichia coli)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …
    Promoter
    T7
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155134-plasmid-map-sequence-id-310516

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  20. rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

    Plasmid
    #122450
    Purpose
    Expression of inositol 1,4,5-trisphosphate 3-kinase A
    Depositor
    Christophe Erneux
    Article
    Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.
    Insert
    inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
    Use
    Tags
    beta-galactosidase
    Expression
    Bacterial
    Mutation
    coding sequence from V66 till stop codon
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    122450-plasmid-map-sequence-id-237990

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Showing: 3341 - 3360 of 5633 results