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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 3341 - 3360 of 14582 results

  2. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205412-plasmid-map-sequence-id-416556

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  6. pEYFP-N1-eKR2

    Plasmid
    #115337
    Purpose
    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse
    Depositor
    Peter Hegemann
    Publication
    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eKR2
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115337-plasmid-map-sequence-id-222075

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  8. pAAV-i53-DM

    Plasmid
    #92171
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
    Depositor
    Daniel Durocher
    Publication
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53-DM
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A, P69L, and L70V mutations and no…
    Promoter
    CMV
    Available since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92171-plasmid-map-sequence-id-182616

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  9. pG1AK

    Plasmid
    #71736
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Publication
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71736-plasmid-map-sequence-id-130104

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  13. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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  14. Plasma membrane-targeted optodPLD

    Plasmid
    #140061
    Purpose
    Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression
    Depositor
    Jeremy Baskin
    Publication
    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phospholipase D
    Tags
    CAAX, CIBN, CRY2, P2A, and mCherry
    Expression
    Mammalian
    Mutation
    H170A (catalytic dead)
    Available since
    March 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140061-plasmid-map-sequence-id-275751

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  16. GFP-V96 ELP

    Plasmid
    #67853
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues
    Depositor
    Andrew MacKay
    Publication
    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP fused to ELP (VPGVG) repeated 96 times
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Promoter
    T7
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67853-plasmid-map-sequence-id-117808

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  18. Cas12Max

    Plasmid
    #195334
    Purpose
    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon-optimized Cas12Max
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195334-plasmid-map-sequence-id-383108

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Showing: 3341 - 3360 of 14582 results