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Showing: 3361 - 3380 of 27842 results

  1. pBI121::chitRatioGFP:LAAQ

    Plasmid
    #240451
    Purpose
    Expression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available Since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240451-plasmid-map-sequence-id-485323

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  3. pDONOR-MARINA

    Plasmid
    #223328
    Purpose
    DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    MARINA
    KIR2.1 channel Golgi-to-plasma membrane trafficking signal
    Use
    CRISPR and Synthetic Biology
    Tags
    mCherry and super-ecliptic pHluorin
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223328-plasmid-map-sequence-id-447745

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  4. pTRE3G-mouse cGASdeltaN-GFP

    Plasmid
    #186878
    Purpose
    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Mouse cGAS truncation mutant (148-508 a.a.) with C-terminal GFP fusion (Cgas Synthetic, Mouse)
    Use
    Retroviral
    Tags
    GFP
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186878-plasmid-map-sequence-id-366111

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  7. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid
    #223166
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Article
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223166-plasmid-map-sequence-id-442868

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  9. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

    Plasmid
    #129050
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA10 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129050-plasmid-map-sequence-id-256225

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  11. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Expression
    Mammalian
    Available Since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112669-plasmid-map-sequence-id-229038

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  13. pIL6-PMEL-hbb

    Plasmid
    #239868
    Purpose
    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239868-plasmid-map-sequence-id-477826

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  14. pPMEL-hbb

    Plasmid
    #239863
    Purpose
    Template for in vitro transcription of PMEL (gp100), including native signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence including native signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239863-plasmid-map-sequence-id-477836

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  16. AAV-KrasG12A/sgKras/Cre

    Plasmid
    #99849
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99849-plasmid-map-sequence-id-193789

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  17. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

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  18. DPAP1-COMP-blac-flag-his

    Plasmid
    #111019
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    cathepsin C, homolog,dipeptidyl aminopeptidase 1 (DPAP1) (PF11_0174 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111019-plasmid-map-sequence-id-224593

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  19. AAV-KrasG13S/sgKras/Cre

    Plasmid
    #99859
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    99859-plasmid-map-sequence-id-200267

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Showing: 3361 - 3380 of 27842 results