We narrowed to 30,244 results for: PLE

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pEMS2046

Plasmid

#79654

Purpose

ssAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple257-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2047

Plasmid

#79655

Purpose

ssAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple258-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG1_UL136_26kDa_Myc

Plasmid

#74955

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

Expression

Mammalian

Mutation

delta (1-62) M78A M80A M100A M128A

Promoter

CMV, Lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG1_UL136_33kda_Myc

Plasmid

#74957

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

Myc tag (EQKLISEEDL)

Expression

Mammalian

Mutation

M63A M78A M80A M100A M128A

Promoter

CMV, Lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2025

Plasmid

#49132

Purpose

AAV plasmid with NOV (Ple303) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple303-iCre

Use

AAV

Expression

Mammalian

Promoter

NOV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

Plasmid

#65847

Purpose

Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

R5D-K6D

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

Plasmid

#199390

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Tags

14xHis-Avi-SUMOEu

Expression

Bacterial

Promoter

T5-LacO

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-osTIR1

Plasmid

#207840

Purpose

Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

osTIR
mCherry
mAID_-VHH-GFP4

Tags

3X HA-Tag and weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Promoter

polyhedrin

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10TEV

Plasmid

#159427

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Tags

TEV-protease-cleavable His10-tag

Expression

Insect

Promoter

polyhedrin

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-TRIM21

Plasmid

#207838

Purpose

Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

TRIM21 (TRIM21 Human)
mCherry
hIgG1-FC
VHH-GFP4

Tags

weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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