We narrowed to 6,508 results for: nls
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Plasmid#193070Purposedoxycycline-inducible expression of mouse Gata2 without the nuclear localisation signal (amino acids 380-440), in mammalian cellsDepositorInsertmCh-Gata2-del-NSL(380-440)
UseLentiviralExpressionMammalianPromoterminimal CMVAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmROSA26-attP50-Neo-mKate2-3xNLS-attP50
Plasmid#183609PurposeTargeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.DepositorInsertsNeo
mKate2-3xNLS
UseMouse TargetingTags3xNLSAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188773PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)
Plasmid#160044PurposeFor expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)DepositorAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9
Plasmid#229773PurposeExpresses Cas9 driven by a CMV promoterDepositorInsertSpCas9
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
dCas9-AsLOV2x3-NLSx3
Plasmid#231566PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE0ΔiNLS
Plasmid#107684PurposeExpresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cellsDepositorInsertdLbCpf1-BE0ΔiNLS
ExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKikGR-PCNA-19-SV40NLS-4
Plasmid#57328PurposeLocalization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593DepositorAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ezh2[SET]-dCas9
Plasmid#100087PurposeCatalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertEzh2[SET] (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[SET]-dCas9-NLSExpressionMammalianMutationEzh2[SET] includes aa 482-746PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213936-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_dTomato_nlsdTomato (#213936). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorTagsdTomatoAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianPromoterCMVAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
T7_CC_PE7_IVT_Template
Plasmid#223022PurposeTemplate for in vitro transcription of PE7. For HSPC-related experiments.DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSPromoterT7Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->GssGFP-P2A-NLSIFP
Plasmid#162235PurposeLentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cellsDepositorInsertGFP - IFP
UseLentiviralExpressionMammalianAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol1-uas-mAKT-2a-nlstdTomato
Plasmid#67706PurposeTol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomatoDepositorInsertmAKT-2A-nlstdTomato
Available SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-MKIIK11A::nlsLexADBDo
Plasmid#64726Purposegenerating transgenic TRIC fliesDepositorInsertMKIIK11A::nlsLexADBDo
ExpressionInsectAvailable SinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-PCNA-19-SV40NLS-4
Plasmid#57154PurposeLocalization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
RPL25NLS-GFP-PRA (102)
Plasmid#24041DepositorInsertRPL25 (RPL25 Budding Yeast)
TagsEGFP and PRAExpressionYeastMutationOnly bp 1-135 (aa 1-45) of RPL25Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9(1.1)-2×sgRNA (IFT88, donor)
Plasmid#80769PurposeExpresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).DepositorAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only