We narrowed to 12,317 results for: 112

1. pLL3 sgRNA(MS2)-MS2-AID3f

   Plasmid

   #112129

   Purpose

   empty sgRNA cloning vector with MS2-AID3f

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AIDmono 3f mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL2 sgRNA(MS2)-MS2-AID3c

   Plasmid

   #112128

   Purpose

   empty sgRNA cloning vector with MS2-AID3c

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono 3c mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUNB.1.0-gDNA

   Plasmid

   #112409

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

   Depositor

   Kevin White

   Insert

   JUNB (JUNB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCON1-ProC.gfp

   Plasmid

   #112517

   Purpose

   ProC driven gfpmut3 expression with synTT

   Depositor

   Olivia Steele-Mortimer

   Article

   Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

   Insert

   ProC gfpmut3 TT
   

   Expression

   Bacterial

   Promoter

   ProC

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5 GFP RagB Q99L

   Plasmid

   #112749

   Purpose

   express GFP-RagB (Q99L mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   GFP-RagB (Q99L) (RRAGB Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q99L

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-GST-RNMT

   Plasmid

   #112709

   Purpose

   Expresses N-terminally GST-tagged human RNMT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Tags

   GST and thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. RhoA sensor (mScarlet-I_10_LSS-SGFP2_10)

   Plasmid

   #112952

   Purpose

   RhoA FRET biosensor

   Depositor

   Dorus Gadella

   Insert

   RhoA sensor
   

   Tags

   LSS-SGFP2 and mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-VHC

   Plasmid

   #112618

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG-FLEX.iGABASnFR

   Plasmid

   #112166

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZ:F3-P CAGGS tdTPH-F

    Plasmid

    #112667

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJW1666

    Plasmid

    #112040

    Purpose

    CEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    None
    

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS-I-Sce1-eno2:Tau-IRES-egfp

    Plasmid

    #112205

    Purpose

    Used to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoter

    Depositor

    Edward Burton

    Article

    Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

    Insert

    MAPT (MAPT Human)
    

    Use

    Zebrafish expression

    Tags

    eno2 exon 2 translational fusion, ires-egfp at c …

    Promoter

    eno2 from zebrafish

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJW1663

    Plasmid

    #112037

    Purpose

    Gal-Estradiol transcription activation expression cassette marked with KanR

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    Gal DNA binding-estradiol binding-MSN2 activator
    

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMito.iGABASnFR.mRuby3

    Plasmid

    #112179

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR
    

    Tags

    mRuby3

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EB3-3xmTq2-N1

    Plasmid

    #112929

    Purpose

    In vivo visualization of microtuble plus ends (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    EB3
    

    Tags

    3x mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1

    Plasmid

    #112261

    Purpose

    Donor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.

    Depositor

    Zhili Rong

    Article

    Ma et al FEBS Lett. 2018 Jun 5. doi: 10.1002/1873

    Insert

    spCas9-p300 (EP300 Synthetic, Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112214

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Mutation

    D10A,H840A,N863A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid

    #112031

    Purpose

    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    

    Tags

    Split mCherry

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOP3MT

    Plasmid

    #112601

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    Avi - 8His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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