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We narrowed to 1,088 results for: control gRNA

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Showing: 321 - 340 of 1088 results

  7. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  10. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse TTN for activation
    Expression
    Mammalian
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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  12. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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  15. pSR3036

    Plasmid
    #222629
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting yejL (b2187) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting yejL
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available Since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222629-plasmid-map-sequence-id-441106

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  16. pSR3039

    Plasmid
    #222630
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting ompF (b0929) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting ompF
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available Since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222630-plasmid-map-sequence-id-441107

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  17. pSR3030

    Plasmid
    #222627
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting ompR (b3405) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting ompR
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available Since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222627-plasmid-map-sequence-id-441108

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  18. pSR3034

    Plasmid
    #222628
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting lapC (b2188) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting lapC
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available Since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222628-plasmid-map-sequence-id-441113

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  19. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human TTN for activation
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128323-plasmid-map-sequence-id-255024

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  20. pSC4

    Plasmid
    #91182
    Purpose
    T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    gRNAs targeting 58kb region in medicago truncatula
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91182-plasmid-map-sequence-id-182722

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Showing: 321 - 340 of 1088 results