We narrowed to 4,236 results for: erf

1. pKD239-1g-2

   Plasmid

   #90959

   Purpose

   Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Tetrathionate response regulator
   Superfolder GFP
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23100, Bba_J23109, and PttrB185-269

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Use

   Tags

   3x-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BAS286 C terminal SF-GFP hygBR

   Plasmid

   #74081

   Purpose

   S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   super-folder GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. SadCas9 KRAB

   Plasmid

   #188504

   Purpose

   Expresses FLAG tagged SadCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-hfYFP

   Plasmid

   #191201

   Purpose

   Constitutive CAG-driven expression of hfYFP in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CAG

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

   Plasmid

   #167902

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Promoter

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

   Plasmid

   #84245

   Purpose

   Expresses direct fusion KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   KRAB-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, KRAB, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKD184

    Plasmid

    #90953

    Purpose

    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23114, PltetO-1, and PphsA

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Burden Monitor phi80 version

    Plasmid

    #66074

    Purpose

    The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-sfGFP-DogTag Loop A

    Plasmid

    #171779

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Val2…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHW450

    Plasmid

    #198831

    Purpose

    Voltage-sensitive fluorescent protein.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD233.7-3

    Plasmid

    #90955

    Purpose

    Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23114, PltetO-1, and PttrB185-269

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSR62

    Plasmid

    #125076

    Purpose

    Expression of ompR under PLtetO-1, output promoter PompF146

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR (ompR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ233 (dLbCpf1-SRDX)

    Plasmid

    #86211

    Purpose

    Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR

    Tags

    5' and 3' NLS and SRDX repressor

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9-beta

    Plasmid

    #169240

    Purpose

    Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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