We narrowed to 2,346 results for: puc19

1. p223-Pulse-Switch

   Plasmid

   #217889

   Purpose

   Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. NK811

   Plasmid

   #196408

   Purpose

   Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

   Plasmid

   #188272

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   AaCas12b(Q119F/E475R/E758R)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

   Plasmid

   #188274

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   PlmCasX(T26R/K610R/K808R)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPG-GPD-TRPC

   Plasmid

   #190113

   Purpose

   Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pILGFP4D41

   Plasmid

   #185841

   Purpose

   Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIPKAH

   Plasmid

   #185869

   Purpose

   Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP10CF72 (B11)

   Plasmid

   #185859

   Purpose

   Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP10CF72 (B9)

   Plasmid

   #185858

   Purpose

   Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP10CF72

    Plasmid

    #185857

    Purpose

    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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