We narrowed to 9,663 results for: BLI

1. pET22B_PcrA_2Bchimera

   Plasmid

   #102998

   Purpose

   Express a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA

   Depositor

   Martin Webb

   Insert

   PcrA_2Bchimera
   

   Expression

   Bacterial

   Mutation

   Bacilllus stearothermophilus PcrA in which the 2B…

   Promoter

   T7

   Available Since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJR255

   Plasmid

   #78549

   Purpose

   For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.

   Depositor

   John Rossi

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   U6, CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCP-tag (m) Vector

   Plasmid

   #101127

   Purpose

   Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Tags

   MCP-tag (Mutant ACP- tag)

   Expression

   Mammalian

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBEST-PLtetO-1-UTR1-TetR-deGFP-T500

   Plasmid

   #45774

   Depositor

   Richard Murray

   Insert

   tetR-deGFP
   

   Use

   Synthetic Biology

   Tags

   Fusion protein of TetR and deGFP, with glycine-se…

   Expression

   Bacterial

   Promoter

   PLtetO-1

   Available Since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9CR4-NG

   Plasmid

   #120225

   Purpose

   pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2-Rad21-N

   Plasmid

   #66951

   Purpose

   Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   Rad21 (RAD21 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pUS252L

   Plasmid

   #191832

   Purpose

   Expresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbones

   Depositor

   Nicholas Coleman

   Insert

   fuBFP
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.3_SARS2_EG.5.1

   Plasmid

   #207641

   Purpose

   pseudovirus plasmid that expresses spike of EG.5.1

   Depositor

   David Nemazee

   Insert

   Spike (EG.5.1) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   Q52H, F456L on the basis of XBB.1.5

   Promoter

   CMV

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos3.2-Vinculin-N-21

   Plasmid

   #66952

   Purpose

   Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   Vinculin (VCL Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   V234L in Vinculin

   Promoter

   CMV

   Available Since

   July 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCP-GPI Control Plasmid

    Plasmid

    #101132

    Purpose

    Expression of MCP-Tagged GPI anchor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    GPI
    

    Tags

    MCP-tag (Mutant ACP- tag)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pet15_rbsk

    Plasmid

    #220227

    Purpose

    Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    ribokinase (rbsK E.coli)
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. miR-124

    Plasmid

    #182363

    Purpose

    Plasmid with 4x miR-124 target sites

    Depositor

    Mario Capecchi

    Insert

    4x miR-124 target sites
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-S-B.1.617.2-FLAG

    Plasmid

    #177097

    Purpose

    B.1.617.2 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.617.2 (S SARS-CoV-2)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 2, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CMV-H2B-sfGFP-T2A-iCre

    Plasmid

    #183351

    Purpose

    Histone H2B-sfGFP and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    Histone H2B-sfGFP-T2A-iCre
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK 3xFLAG-NUP37 neo

    Plasmid

    #220361

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged NUP37

    Depositor

    Kevin Janes

    Insert

    NUP37 (NUP37 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p221-RV-dlx-dio-Tdtomato

    Plasmid

    #185697

    Purpose

    Retroviral vector expression cre-dependent membrane tagged Tdtomato driven by the mDlx promoter

    Depositor

    Lynette Lim

    Insert

    membrane Tdtomato
    

    Use

    Cre/Lox and Retroviral

    Tags

    Gap43 palmitoylation

    Promoter

    mDlx

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-FLEX-TVA66T-EGFP

    Plasmid

    #64091

    Purpose

    Expresses TVA66T-EGFP under the CAG promotor

    Depositor

    Ian Wickersham

    Insert

    TVA66T-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glutamic acid 66 to Threonine

    Promoter

    CAG

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHflu2

    Plasmid

    #203880

    Purpose

    pHI1 with insertion of ampicillin-resistance gene

    Depositor

    Bogumil Karas

    Insert

    broad-spectrum class A beta-lactamase TEM-116
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4MVT

    Plasmid

    #60101

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIAS3:YTC008-B10:C225910 (PIAS3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits