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pBAD18HMGR

Plasmid

#19041

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pJKW0776

Plasmid

#119024

Purpose

E.coli expression plasmid for PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmSPS2

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW0826

Plasmid

#119027

Purpose

E.coli expression plasmid for PmKOMT2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmKOMT2

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJKW1131

Plasmid

#119028

Purpose

E.coli expression plasmid for PmKLR1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmKLR1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRG221

Plasmid

#64520

Purpose

Episomal E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJKW1574

Plasmid

#119178

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS1

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS1

Expression

Bacterial

Promoter

pGAP

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-oROS-HT-CaaX

Plasmid

#216417

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT-CaaX

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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ALIX-His(1-868, P801G)

Plasmid

#186808

Purpose

Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

ALIX-His (1-868, P801G) (PDCD6IP Human)

Tags

His6

Expression

Bacterial

Mutation

Proline 801 to Glycine

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

Human ferritin

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nprot

Plasmid

#157729

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nprot (N SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CNNM3-CBS-YPet

Plasmid

#149686

Purpose

For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

Depositor

Motoyuki Hattori

Article

Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

Insert

CNNM3-CBS-YPet (CNNM3 Synthetic, Human)

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

Plasmid

#132682

Purpose

AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

AAV

Tags

6xHIS and Flag

Promoter

Chicken b-actin

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

Plasmid

#132685

Purpose

AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 2 (Cndp2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-3xHA-V5-RpL10Ab

Plasmid

#125223

Purpose

3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

3x HA, V5

Expression

Insect

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-L11

Plasmid

#127831

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MBD3L2

Plasmid

#106332

Purpose

All-in-one lentivector with tet-inducible MBD3L2

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-AMVpl-δWT-nAchR

Plasmid

#97188

Purpose

To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

Delta nicotinic acetylcholine receptor (Chrnd Mouse)

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P2 fragment)

Plasmid

#86444

Purpose

Human ABCA1 promoter luciferase reporter (P2 fragment)

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P3 fragment)

Plasmid

#86445

Purpose

Human ABCA1 promoter luciferase reporter (P3 fragment)

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Idax DBM

Plasmid

#68554

Purpose

This plasmid expresses full length IDAX containing a DNA binding mutation.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

IDAX-DBM (Cxxc4 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

T162A, H164A and Q165A mutations

Promoter

EF1a

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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