We narrowed to 7,394 results for: 11

1. MTK678_002

   Plasmid

   #123923

   Purpose

   Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BAC-AmpR
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMKO.1-shMki67

   Plasmid

   #160958

   Purpose

   Mki67 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Mki67 (Mki67 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP-Stim1-D76A

   Plasmid

   #186622

   Purpose

   Encodes Stim1 mutant D76A fused to YFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Stromal Interaction Molecule 1 (STIM1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D76A

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-Dusp6_2KBpromoter

   Plasmid

   #32666

   Depositor

   Michael Tsang

   Article

   Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

   Insert

   Dusp6 (dusp6 Zebrafish)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. 375 pME-fli1a

   Plasmid

   #49001

   Purpose

   full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   friend leukemia integration 1a (fli1 Zebrafish)
   

   Use

   Gateway middle entry

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB3050(gRNA XII-5)

   Plasmid

   #73292

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK2_008

   Plasmid

   #123703

   Purpose

   Encodes human pUbc as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   phUbC
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS/U6/RNAi SadBs shRNA mouse

   Plasmid

   #67152

   Purpose

   pBS/U6 containing shRNA to mouse SADBs

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   BRSK1 (Brsk1 Mouse)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSuper.Retro.Puro-WDR5 shRNA 2

   Plasmid

   #59975

   Purpose

   Knockdown of WDR5

   Depositor

   Debu Chakravarti

   Article

   Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

   Insert

   WDR5 shRNA
   

   Use

   Retroviral

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pONSY-CoH2B:Venus

    Plasmid

    #111877

    Purpose

    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora Histone H2B (CoH2B) fused to Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sha2Cas9-HF

    Plasmid

    #192142

    Purpose

    Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Sha2Cas9-HF (R247A and N415A)

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXA9 (murine) FLAG pRC/CMV

    Plasmid

    #8514

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    FLAG epitope tag replaces T7 tag in the pET back…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. hSTARR-seq_SCP1 vector_blocking 3

    Plasmid

    #99318

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB3047(gRNA XII-1)

    Plasmid

    #73289

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBlastR-MCS-QF-MiniWhite

    Plasmid

    #165906

    Purpose

    Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. JPF0512

    Plasmid

    #124003

    Purpose

    Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK3_027

    Plasmid

    #123742

    Purpose

    Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    VP16::PYLcs-T2A-GAL4DBD::ABIcs
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

    Plasmid

    #56658

    Purpose

    expresses constitutively active human PKCi with a deletion on the PB1 domain

    Depositor

    Pedro Salas

    Article

    Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

    Insert

    Human PKCi (PRKCI Human)
    

    Use

    Lentiviral

    Tags

    V5/His

    Mutation

    A120E and deletion of PB1 domain (aa 24–98)

    Promoter

    CMV

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-HA-REDD1 R133A/P139A (#626)

    Plasmid

    #99946

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R133A/P139A

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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