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pSuper.Retro.Puro-PKN1 shRNA 4

Plasmid

#59978

Purpose

Knockdown of PKN1

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

PKN1 shRNA

Use

Retroviral

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (E870K)

Plasmid

#31802

Depositor

Yardena Samuels

Publication

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 870 to lysine

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (S610L)

Plasmid

#31800

Depositor

Yardena Samuels

Publication

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed serine 610 to leucine

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
Flag-hPKL WT

Plasmid

#107159

Purpose

For mammalian expression of Flag-hPKL WT

Depositor

Jesse Rinehart

Publication

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL WT (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [Chronos-tdTomato]

Plasmid

#105834

Purpose

AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2904(XI-3 MarkerFree)

Plasmid

#73276

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.hSynapsin.SF-iGluSnFR.A184S (AAV9)

Viral prep

#106174-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV.hSynapsin.SF-iGluSnFR.A184S (#106174). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.A184S plasmid DNA. hSynapsin driven SF-iGluSnFR.A184S glutamate sensor. The A184S mutant has a higher glutamate affinity. These AAV preparations are suitable purity for injection into animals.

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Promoter

Syn

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only
pQUAST-M13::GAL4DBDo

Plasmid

#64724

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Publication

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

M13::GAL4DBDo

Expression

Insect

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRM430

Plasmid

#44530

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-30

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pUK192

Plasmid

#44962

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

LEU2

Expression

Yeast

Mutation

HHTI-HHF1 is replaced with LEU2

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only
pRM214

Plasmid

#44527

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

K14R

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pRM204

Plasmid

#44528

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pRM415

Plasmid

#44526

Depositor

Michael Grunstein

Publication

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHT2

Expression

Yeast

Mutation

hht2 deletion 4-15

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only
pIC-RFP-1

Plasmid

#254928

Purpose

All-in-one plasmid developed for inducible CRISPRi-assisted genome editing in Streptomyces

Depositor

Gilles van Wezel

Publication

Bai et al Nucleic Acids Res. 2026 Jun 8;54(11):gka

Insert

dCas9

Use

CRISPR and Synthetic Biology

Promoter

SP30

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-CKAR2

Plasmid

#236100

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

Depositor

Jin Zhang

Publication

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

LAMP1-ExRai-CKAR2 (LAMP1 Synthetic, Human)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-ATP

Plasmid

#225247

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

Depositor

Yasushi Okada

Publication

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-ATP

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-citrate

Plasmid

#225251

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

Depositor

Yasushi Okada

Publication

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-citrate

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Publication

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-C1-Nir1-LNS2

Plasmid

#220085

Purpose

high affinity PA biosensor with far-red fluorophore

Depositor

Gerry Hammond

Publication

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

iRFP670-GGSGGM

Expression

Mammalian

Mutation

amino acids 613-897

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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