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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,739 results for: BLI

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Showing: 3421 - 3440 of 9739 results

  1. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  2. SNX32A-c014

    Plasmid
    #175486
    Purpose
    Protein expression in bacterial cells. PX domain, S17-M197
    Depositor
    Opher Gileadi
    Insert
    SNX32 (SNX32 Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175486-plasmid-map-sequence-id-353158

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  3. FLAG-hMYF5 (#795)

    Plasmid
    #78333
    Purpose
    Overexpression of human MYF5 ORF with a N-terminal FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    MYF5 (MYF5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78333-plasmid-map-sequence-id-373227

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  4. pAAV-CAG-mNeonGreen

    Plasmid
    #124101
    Purpose
    Expression of the fluorescent protein mNeonGreen in mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mNeonGreen
    Use
    AAV
    Expression
    Mammalian
    Mutation
    Codon usage was optimized for Homo Sapiens
    Promoter
    CAG
    Available Since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124101-plasmid-map-sequence-id-241203

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  5. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  6. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

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  7. BA.2.86

    Plasmid
    #208583
    Purpose
    Mammalian expression vector for expressing BA.2.86 SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant BA.2.86 spike (S )
    Expression
    Mammalian
    Available Since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208583-plasmid-map-sequence-id-409580

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  8. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

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  9. PRDX4

    Plasmid
    #38851
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PRDX4 (Prdx4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse TTN for activation
    Expression
    Mammalian
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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  11. pEGFPN1‐mTim1

    Plasmid
    #49215
    Purpose
    expresses EGFP tagged mouse Tim1
    Depositor
    Lawrence Kane
    Insert
    Tim1 (Havcr1 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pcDNA3.1-hSyncytin-1(del480)

    Plasmid
    #182441
    Purpose
    hSyncytin-1, deletion 481-538
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Expression
    Mammalian
    Mutation
    deletion 481-538
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182441-plasmid-map-sequence-id-357582

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  13. pAG Retro Puro Empty

    Plasmid
    #234458
    Purpose
    Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.
    Depositor
    Melina Fan, Addgene Research Program
    Type
    Empty backbone
    Use
    Retroviral
    Available Since
    March 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234458-plasmid-map-sequence-id-464639

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  14. PTGR1

    Plasmid
    #38874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTGR1 (PTGR1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. p426TEF-LptTPS-A

    Plasmid
    #89468
    Purpose
    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production
    Depositor
    Jing-Ke Weng
    Insert
    LptTPS-A
    Tags
    none
    Expression
    Yeast
    Promoter
    pBR322 origin of replication for propagation in E…
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89468-plasmid-map-sequence-id-176087

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  16. pACP-tag (m)- 2 Vector

    Plasmid
    #101126
    Purpose
    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of ACP-tag.
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    ACP-tag
    Expression
    Mammalian
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101126-plasmid-map-sequence-id-194174

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  17. pHD-w+

    Plasmid
    #80927
    Purpose
    Vector for generating dsDNA donors for HDR. Empty backbone containing a StuI restriction site and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Insert
    GMR-white
    Use
    High copy, amp resistance
    Promoter
    GMR::hsp70
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80927-plasmid-map-sequence-id-152786

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  18. mT2A-tdTomato-FNF

    Plasmid
    #182340
    Purpose
    Recombineering cassette plasmid for T2A-tdTomato-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-tdTomato-FNF
    Use
    Mouse Targeting; Flp / frt
    Expression
    Mammalian
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182340-plasmid-map-sequence-id-358215

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  19. pNHA_OROV_NCP

    Plasmid
    #208937
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Nucleocapsid
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted start codon so HA included at N-terminus
    Promoter
    CMV
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208937-plasmid-map-sequence-id-418963

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  20. pBHM2647

    Plasmid
    #229989
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229989-plasmid-map-sequence-id-461139

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Showing: 3421 - 3440 of 9739 results