We narrowed to 3,866 results for: mak

1. pDZ645 pKAN 1x-mCherry-12xPP7 V4

   Plasmid

   #73173

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   1x-mCherry
   

   Expression

   Mammalian and Yeast

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLLX13

   Plasmid

   #79825

   Purpose

   Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

   Depositor

   Stephen Lory, Cammie Lesser

   Article

   Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Type

   Empty backbone

   Use

   Yeast and bacteria shuttle vector, contains origi…

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYIAL31

   Plasmid

   #60807

   Purpose

   used to make mutations in yeast POL1

   Depositor

   Thomas Kunkel, Youri Pavlov

   Article

   Pavlov et al Genetics. 2001 Sep;159(1):47-64.

   Insert

   Pol1 (POL1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   N-terminal part only

   Available Since

   Feb. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-HD118

   Plasmid

   #172655

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD118A/pegRNA-HD118B
   

   Use

   CRISPR

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HOXA9 (murine) HIS-tag pET

   Plasmid

   #8492

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pLLX8

   Plasmid

   #79838

   Purpose

   Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

   Depositor

   Stephen Lory, Cammie Lesser

   Article

   Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Type

   Empty backbone

   Use

   Contains yeast counter selection gene, cyh2 and b…

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG mIgG2a Anti-c-Myc [9E10]

   Plasmid

   #242955

   Purpose

   Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain constant region; to be used with pAG mKappa Anti-c-Myc light chain [9E10] (Plasmid 242956) to make the antibody.

   Depositor

   Meghan Rego, Addgene Research Program

   Insert

   Anti-c-Myc [9E10] fused to mouse IgG2a heavy chain
   

   Tags

   Mouse IgG2a

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pAG mKappa Anti-c-Myc [9E10]

   Plasmid

   #242956

   Purpose

   Mammalian expression plasmid for Anti-c-Myc [9E10] fused to mouse kappa light chain constant region; to be used with pAG mIgG2a Anti-c-Myc [9E10] heavy chain (Plasmid 242955) to make the antibody.

   Depositor

   Meghan Rego, Addgene Research Program

   Insert

   Anti-c-Myc [9E10] light chain variable region fused to mouse kappa light chain constant region
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT

   Plasmid

   #242750

   Purpose

   Expresses unspliced EPB41L4A-AS1 in mammalian cells

   Depositor

   Igor Ulitsky

   Article

   Monziani et al bioRxiv 2025.04.08.647803

   Insert

   EPB41L4A-AS1 (EPB41L4A-AS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

   Promoter

   CMV

   Available Since

   Sept. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIVT-evoBxb1

    Plasmid

    #232747

    Purpose

    IVT template for making modRNA encoding evoBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Mutation

    V74A

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIVT-Bxb1-I87L

    Plasmid

    #232749

    Purpose

    IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Mutation

    I87L

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST17-HO1(51-231)

    Plasmid

    #203486

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Arabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
    

    Expression

    Bacterial and Plant

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lentiguide-Blast-eNMU710

    Plasmid

    #172658

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-eNMUA/pegRNA-eNMUB
    

    Use

    CRISPR

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCsB_spacer

    Plasmid

    #136076

    Purpose

    Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCsA_spacer

    Plasmid

    #136075

    Purpose

    Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. PB-HD252

    Plasmid

    #172656

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD252A/pegRNA-HD252B
    

    Use

    CRISPR

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-APEX2 (GC rich)

    Plasmid

    #115550

    Purpose

    Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    APEX2 (GC rich)
    

    Use

    Multisite gateway entry clone

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIVT/Acrosin-GFP-GPI

    Plasmid

    #122141

    Purpose

    For making acrosin-GFP-GPI RNA

    Depositor

    Carmen Williams

    Article

    Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

    Insert

    acrosin-GFP-GPI
    

    Use

    For in vitro transcription

    Promoter

    T7

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-6NBC-GFP-PGK-Puro-Barcode9

    Plasmid

    #83686

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode9
    

    Use

    Retroviral

    Mutation

    please view depositor comments below

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-6NBC-GFP-PGK-Puro-Barcode7

    Plasmid

    #83684

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode7
    

    Use

    Retroviral

    Mutation

    L119P in GFP

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge