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pMB.BIG1a

Plasmid

#228675

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.008

Plasmid

#228674

Purpose

tSV40 Terminator for Baculovirus expression with Cterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

tSV40

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.006

Plasmid

#228672

Purpose

polH Promoter for Baculovirus expression with Nterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

polH

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.003

Plasmid

#228669

Purpose

Recombination to BV DNA (Tn7)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Tn7L

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.002

Plasmid

#228668

Purpose

Moclo Baculo toolkit level 2 backbone

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.001

Plasmid

#228667

Purpose

Moclo Baculo toolkit level 1 backbone

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-12)

Plasmid

#219420

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-12)

Tags

MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVN

Plasmid

#223861

Purpose

4G-cloning donor for mammalian promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-GST-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-MBP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-HA2-TEV

Plasmid

#223876

Purpose

4G-cloning donor for N-terminal His6-HA2-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-HA2-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3-TEV

Plasmid

#223875

Purpose

4G-cloning donor for N-terminal His6-FLAG3-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-FLAG3-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phC

Plasmid

#223859

Purpose

4G-cloning donor for insect promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-Venus-His8

Plasmid

#223889

Purpose

4G-cloning donor for C-terminal 3C-Venus-His8 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-Venus-His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVC

Plasmid

#223862

Purpose

4G-cloning donor for mammalian promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_TUB4S351F_1397NC

Plasmid

#193382

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtTUB4.

Expression

Plant

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-tdTomato-sg

Plasmid

#194722

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-GFP-sg

Plasmid

#194717

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mApple_pcDNA3.1+

Plasmid

#182855

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mApple

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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