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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 3421 - 3440 of 6562 results

  1. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  2. pSmart-EF1a-Nla-cas5

    Plasmid
    #178878
    Purpose
    Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas5c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178878-plasmid-map-sequence-id-351751

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  3. pSmart-EF1a-Nla-cas7

    Plasmid
    #178879
    Purpose
    Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas7c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178879-plasmid-map-sequence-id-351739

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  4. pSmart-EF1a-Nla-cas8

    Plasmid
    #178880
    Purpose
    Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas8c-bGH PolyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178880-plasmid-map-sequence-id-351750

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  5. pSmart-EF1a-Nla-cas11

    Plasmid
    #178882
    Purpose
    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a-NlaCas11c-bGH polyA
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178882-plasmid-map-sequence-id-351753

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  6. CIB-VP16 (B1014)

    Plasmid
    #92036
    Purpose
    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.
    Depositor
    Chandra Tucker
    Publication
    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIB1 (CIB1 Mustard Weed)
    Tags
    Fusion of plant CIB1 (intact NLS) with VP16 activ…
    Expression
    Mammalian
    Promoter
    T7
    Available since
    July 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    92036-plasmid-map-sequence-id-183932

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  7. pY095

    Plasmid
    #84744
    Purpose
    Expresses huLbCpf1-T2A-GFP and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huLbCpf1
    GFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    84744-plasmid-map-sequence-id-196925

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  9. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid
    #158160
    Purpose
    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-3xHA-miniTurbo-3XNLS
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158160-plasmid-map-sequence-id-309971

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  11. SpRY-ABE8e(V106W)

    Plasmid
    #198553
    Purpose
    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpRY-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198553-plasmid-map-sequence-id-388905

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  12. SpCas9-NG-YE1-BE4max

    Plasmid
    #198535
    Purpose
    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198535-plasmid-map-sequence-id-388892

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  15. Lenti-LbCpf1-Blast

    Plasmid
    #84751
    Purpose
    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    LbCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84751-plasmid-map-sequence-id-164465

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  17. pCMV-ABE8e-NRCH

    Plasmid
    #165416
    Purpose
    Expresses the base editor ABE8e-NRCH in mammalian cells
    Depositor
    David Liu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE8e-NRCH
    Tags
    BP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    165416-plasmid-map-sequence-id-334022

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Showing: 3421 - 3440 of 6562 results