We narrowed to 31,305 results for: son

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mKO-pBAD

Plasmid

#54735

Purpose

Localization: Protein Expression Vector , Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and mKO

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf_pvc13-FLAG

Plasmid

#198297

Purpose

Produces PVCs retargeted with FLAG tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-FLAG

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphaoB-RLuc8

Plasmid

#140977

Purpose

Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphaoB-Rluc8 (GNAO1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

Plasmid

#78353

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1

Plasmid

#119855

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE19

Plasmid

#90361

Purpose

Wnt - TCF/LEF gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

Wnt

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHN922

Plasmid

#178975

Purpose

Expression of GST-His8-GFP-nanobody

Depositor

Kazuhiro Abe

Insert

GST-His8-GFP-nanobody

Use

Affinity Reagent/ Antibody

Tags

GST, His8

Expression

Bacterial

Promoter

tac

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEVp

Plasmid

#162599

Purpose

TEV protease under CMV promoter

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

TEV protease

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Lifeact_Halo_T2A_EGFP

Plasmid

#135445

Purpose

Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

Lifact-HaloTag7 and EGFP

Tags

Lifact

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-mCherry

Plasmid

#127887

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ150-Cas9(dpiRNA)

Plasmid

#107940

Purpose

eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

Cas9(dpiRNA)

Expression

Worm

Promoter

eft-3

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-CathespinB-6

Plasmid

#55007

Purpose

Localization: Lysosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CathespinB

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-E-Cadherin-N-18

Plasmid

#54072

Purpose

Localization: Cadherin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

E-Cadherin

Tags

mEmerald

Expression

Mammalian

Mutation

T143A, D684Y

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pTmpl-miniDonor-GFP

Plasmid

#238107

Purpose

Mini donor expressing GFP, used for PCR to generate IVT template.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHCMV-VSVgSS-15F11-TM

Plasmid

#207317

Purpose

Functional incorporation of 15F11 scFv (anti-HA) into envelope of virions

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

15F11 ScFv

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE48

Plasmid

#90387

Purpose

IRF3/IRF7 - viral response gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF3/IRF7

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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