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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 47,759 results for: vit;

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Showing: 5161 - 5190 of 47759 results

  1. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

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  2. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid
    #234439
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234439-plasmid-map-sequence-id-465689

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  3. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234454
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234454-plasmid-map-sequence-id-465691

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  9. pcDNA3.1-GSDMD_29-484

    Plasmid
    #218231
    Purpose
    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-484
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218231-plasmid-map-sequence-id-436177

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  10. pcDNA3.1-GSDMD_29-270

    Plasmid
    #218232
    Purpose
    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218232-plasmid-map-sequence-id-430566

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  11. Gs(short)-CASE

    Plasmid
    #168124
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG1
    Expression
    Mammalian
    Mutation
    NLuc is inserted at N136/V137 within GNAS
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168124-plasmid-map-sequence-id-332868

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  12. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  17. pOLENTE

    Plasmid
    #80683
    Purpose
    DEST vector for cell-free in vitro expression
    Depositor
    Nico Dissmeyer
    Type
    Empty backbone
    Use
    Synthetic Biology; In vitro transcription and tra…
    Expression
    Mammalian
    Promoter
    T7 and SP6
    Available Since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    80683-plasmid-map-sequence-id-152045

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  18. pET26b-BsaI

    Plasmid
    #233523
    Purpose
    Purification vector with BsaI cut sites for streamlined cloning
    Depositor
    Carl Denard
    Type
    Empty backbone
    Tags
    6xHis-tag and pelB leader
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233523-plasmid-map-sequence-id-474147

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  19. pIVTR(T7G)-NS1-TX91

    Plasmid
    #172289
    Purpose
    In vitro transcription of NS1 Influenza A virus protein (strain Texas91) for enhanced in vitro transcribed mRNA translation
    Depositor
    Patrik Verstreken
    Insert
    NS1-TX91
    Use
    Other
    Promoter
    T7 promoter G-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172289-plasmid-map-sequence-id-340308

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  20. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

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  21. pCR175

    Plasmid
    #240640
    Purpose
    Template for in vitro transcription of mTurquoise2 mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    mTurquoise2
    Use
    Vertebrate gene expression and general in vitro t…
    Promoter
    SP6
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240640-plasmid-map-sequence-id-483016

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  22. pCR178

    Plasmid
    #240641
    Purpose
    Template for in vitro transcription of mScarlet-I mRNA using SP6 polymerase.
    Depositor
    John Pringle
    Insert
    mScarlet-I
    Use
    Vertebrate gene expression and general in vitro t…
    Promoter
    SP6
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240641-plasmid-map-sequence-id-482342

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  25. SDC4A-c001

    Plasmid
    #205119
    Purpose
    SDC4 protein expression
    Depositor
    Paul Brennan
    Insert
    SDC4 (SDC4 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205119-plasmid-map-sequence-id-411015

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  26. pN4

    Plasmid
    #233521
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1-10
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233521-plasmid-map-sequence-id-471179

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Showing: 5161 - 5190 of 47759 results