We narrowed to 13,224 results for: CRISPR-Cas9

1. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212830

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212831

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

   Plasmid

   #121818

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 and H1

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)

   Viral Prep

   #112677-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

6. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

   Viral Prep

   #112677-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)

   Viral Prep

   #112677-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

   Viral Prep

   #112677-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

9. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)

   Viral Prep

   #112677-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Promoter

   EF1a

   Tags

   mCherry (Cre-negative cells), EGFP (Cre-positive cells)

   Available since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

10. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

    Viral Prep

    #112677-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-BD10-SAS620-3’dCas9-VPR-synpA

    Plasmid

    #216323

    Purpose

    Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cas9-VPR + splice acceptor site
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

    Plasmid

    #216322

    Purpose

    Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid

    #199221

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    tight TRE promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid

    #199218

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    CMV D2ir promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE

    Plasmid

    #87912

    Purpose

    Kamicas9 lentiviral vector

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    shHTT1, sgCas9, cherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1, 7SK, PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

    Plasmid

    #209292

    Purpose

    Expresses R2Bm-Cas9(H840A) fusion in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Use

    Tags

    His, MBP, SpCas9(H840A), StrepII, and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2-sgCTRL

    Plasmid

    #209750

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI

    Plasmid

    #126480

    Purpose

    Expresses Cas9-GSAlinker-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    GSA linker
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed codons to reduce repetitive sequences.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

    Plasmid

    #126477

    Purpose

    Expresses Cas9-5xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    5xFlag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed codons to reduce repetitive sequences.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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