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pBABE puro Tusc5

Plasmid

#87952

Purpose

retroviral plasmid containing mouse Tusc5

Depositor

David James

Article

Fazakerley et al J Biol Chem. 2015 Sep 25;290(39):23528-4

Insert

Tumor Suppressor Candidate 5 (Tusc5 Mouse)

Use

Retroviral

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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NPRL2_pLX307

Plasmid

#98364

Purpose

Lentiviral expression of NPRL2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

NPRL2 (NPRL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF8_HA_BASU

Plasmid

#153982

Purpose

Express Orf8 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf8 (ORF8 Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF1-V5-miniTurbo

Plasmid

#207810

Purpose

C-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF1-V5-miniTurbo (NFE2L1 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Promoter

UBC

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF1

Plasmid

#207809

Purpose

N-terminal tagged NRF1 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF1 (NFE2L1 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Promoter

UBC

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp14_HA_BASU

Plasmid

#153993

Purpose

Express NSP14 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

NSP14 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA2

Plasmid

#82544

Purpose

Overexpression vector for Venus-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Tags

Venus fluorescent protein

Expression

Mammalian

Promoter

chicken beta actin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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TRAPPC8_pLX307

Plasmid

#98375

Purpose

Lentiviral expression of TRAPPC8

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TRAPPC8 (TRAPPC8 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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F2-bio-His

Plasmid

#52179

Purpose

Expresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

F2 (F2 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp9_HA_BASU

Plasmid

#153986

Purpose

Express NSP9 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp5_HA_BASU

Plasmid

#153987

Purpose

Express NSP5 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp5 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_N_HA_BASU

Plasmid

#153989

Purpose

Express N gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS4

Plasmid

#172422

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 4 (TMPRSS4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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SARS_COV2_nsp2_HA_BASU

Plasmid

#153988

Purpose

Express NSP2 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pINTO-NSA::hSNRNP70

Plasmid

#61726

Purpose

Expression of human SNRNP70 fused to monomeric streptavidin (N-terminus)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Insert

SNRP70 (SNRNP70 Human)

Tags

mSA (monomeric streptavidin)

Expression

Mammalian

Mutation

Two silent mutations to ensure restriction site c…

Promoter

T-REx (CMV + 2xTetO2)

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

Plasmid

#90014

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-TNRC6C

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-PNGase-Strep-HA

Plasmid

#113490

Purpose

Expression of human PNGase with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

PNGase (NGLY1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_nsp15_HA_BASU

Plasmid

#153985

Purpose

Express NSP15 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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