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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 3441 - 3460 of 18824 results

  1. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid
    #109426
    Purpose
    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109426-plasmid-map-sequence-id-212924

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  3. LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

    Plasmid
    #47453
    Purpose
    epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE (N136,Grm2,C63)-cib1
    Use
    AAV; Tale
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47453-plasmid-map-sequence-id-72982

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  4. pCMV-BE3-FNLS(RA)

    Plasmid
    #112671
    Purpose
    CMV expression vector for BE3-FNLS construct (codon optimized)
    Depositor
    Lukas Dow
    Publication
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3-FNLS
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    NLS sequence at the N-terminus and D10A
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112671-plasmid-map-sequence-id-217194

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  9. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

    Plasmid
    #100944
    Purpose
    Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)
    Tags
    3xTy1
    Expression
    Mammalian
    Mutation
    F636A, E660A, E725A, R295A
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100944-plasmid-map-sequence-id-206112

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  10. CMV ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid
    #85806
    Purpose
    Genetically encoded voltage sensor ArcLight- codon optimized
    Depositor
    Vincent Pieribone
    Publication
    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ArcLightCo-Q239
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    CMV
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    85806-plasmid-map-sequence-id-170999

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  12. hfDECOR-F148A

    Plasmid
    #219546
    Purpose
    Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hfDECOR-F148A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed TadA8e Phenylalanine 148 to Alanine
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219546-plasmid-map-sequence-id-443106

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  13. hfDECOR-V106G

    Plasmid
    #219547
    Purpose
    Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hfDECOR-V106G
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed TadA8e Valine 106 to Glycine
    Promoter
    CMV
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219547-plasmid-map-sequence-id-443107

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  15. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid
    #61850
    Purpose
    cell cycle marker
    Depositor
    Jonathon Pines
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA (PCNA Human)
    Tags
    NLS and mRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

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Showing: 3441 - 3460 of 18824 results