We narrowed to 6,241 results for: nls

1. PX458-3xHA-dFnCas9

   Plasmid

   #201954

   Purpose

   Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dFnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A and H969A on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-dSpCas9

   Plasmid

   #201953

   Purpose

   Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-dSpCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A and H840A on SpCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCUU

   Plasmid

   #159747

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-Tet-Cas9-BFP-PGK-Blast

   Plasmid

   #196720

   Purpose

   Tet-inducible expression of Cas9-T2A-TagBFP

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-ABE-P48R

   Plasmid

   #161816

   Purpose

   Expresses ABE-P48R in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HaloTag-LacI

   Plasmid

   #232636

   Purpose

   lac repressor (LacI) which binds lacO sites; labeled with HaloTag

   Depositor

   Karsten Rippe

   Insert

   HaloTag-LacI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

   Plasmid

   #196722

   Purpose

   Tet-inducible expression of Cas9-T2A-miRFP670nano

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHM840

   Plasmid

   #20701

   Depositor

   Thomas Stamminger

   Article

   Sorg et al Biotechniques. 1999 May . 26(5):858-62.

   Insert

   GFP-NLS-lacZ
   

   Expression

   Mammalian

   Available Since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pb-CAGGS-dCas9-blasto

    Plasmid

    #218555

    Purpose

    Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance

    Depositor

    Alejandro Chavez

    Insert

    dCas9
    

    Use

    CRISPR; Piggybac transposition

    Tags

    2xSV40NLS

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PHGDH

    Plasmid

    #38841

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid

    #68336

    Purpose

    Campylobacter lari CF89-12 Cas9

    Depositor

    Feng Zhang

    Insert

    ClCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX399 Mycoplasma gallisepticum str. F Cas9

    Plasmid

    #68335

    Purpose

    Mycoplasma gallisepticum str. F Cas9

    Depositor

    Feng Zhang

    Insert

    MgCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX379 Legionella pneumophila str. Paris Cas9

    Plasmid

    #68316

    Purpose

    Legionella pneumophila str. Paris Cas9

    Depositor

    Feng Zhang

    Insert

    LpCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHygRB12

    Plasmid

    #244899

    Purpose

    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attB site for RMHE

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange; recombi…

    Expression

    Worm

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHygRB2

    Plasmid

    #244897

    Purpose

    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attB site for RMHE

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange; recombi…

    Expression

    Worm

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHygGB16

    Plasmid

    #244896

    Purpose

    hygR selection nls-GFP marked rapid RMCE integration vector with an attB site for RMHE

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange; recombi…

    Expression

    Worm

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHyGB6

    Plasmid

    #244894

    Purpose

    hygR selection nls-GFP marked rapid RMCE integration vector with an attB site for RMHE

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange; recombi…

    Expression

    Worm

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHygGP6

    Plasmid

    #244902

    Purpose

    hygR selection nls-GFP marked rapid RMCE integration vector with an attP site for RMHE

    Depositor

    Michael Nonet

    Article

    Nonet et al bioRxiv 2024.03.01.583017

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange; recombi…

    Expression

    Worm

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge