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Showing: 3461 - 3480 of 160434 results

  3. SpCas9-NG-YE1-BE4max

    Plasmid
    #198535
    Purpose
    Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198535-plasmid-map-sequence-id-388892

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  6. pJYS1Ptac

    Plasmid
    #85545
    Purpose
    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac and unknown
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85545-plasmid-map-sequence-id-176966

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  7. pET-BiFC

    Plasmid
    #87856
    Purpose
    pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187
    Inserts
    Encodes for MCS & C-term half of mVenus
    Encodes for MCS & N-term half of mVenus
    Use
    Tags
    BiFC C-terminal fragment
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87856-plasmid-map-sequence-id-175019

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  12. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

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  14. pAAV-hSyn-DIO-mCherry (AAV1)

    Viral Prep
    #50459-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only

  15. C321.ΔA.exp

    Bacterial Strain
    #49018
    Purpose
    This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C
    Depositor
    George Church
    Article
    Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d
    Bacterial Resistance
    Bleocin (zeocin)
    Species
    Escherichia coli
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  20. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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Showing: 3461 - 3480 of 160434 results