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GreenT-EC.b - Display - CyRFP1

Plasmid

#193944

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pXYB089

Plasmid

#239628

Purpose

Plant RNA Vision constructs with no gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01
stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB082

Plasmid

#239578

Purpose

Plant RNA Vision constructs with 41 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA01
gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB083

Plasmid

#239579

Purpose

Plant RNA Vision constructs with 82 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA03
gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pNCS-mCardinal2

Plasmid

#52631

Purpose

bacterial expression vector with far-red FP mCardinal2

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mCardinal2

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPlum-Mito-3

Plasmid

#55988

Purpose

Localization: Mitochondria, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Mito (COX8A Human)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
GreenT-EC.c - Display - CyRFP1

Plasmid

#193945

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-L1T3D

Plasmid

#33276

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L1 (L1, L1 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Jan. 12, 2012

Availability

Academic Institutions and Nonprofits only

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mEmerald-PDHA1-N-10

Plasmid

#54225

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

PDHA1 (PDHA1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pmiRFP703-Tubulin

Plasmid

#79991

Purpose

Alpha tubulin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Tubulin (TUBA1B Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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BC1450-H2B 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)

Plasmid

#164041

Purpose

CRISPR donor plasmid to insert mCherry-CRISPR-TagV2 into the C-terminus of human H2B gene

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

mCherry-CRISPR-TagV2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-KS

Plasmid

#237690

Purpose

For overexpression of 52K-GFP-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-KS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-AlstR-IRES2-EGFP

Plasmid

#14895

Depositor

Edward Callaway

Article

Tan et al Neuron. 2006 Jul 20. 51(2):157-70.

Insert

AlstR (AstA-R1 Fly)

Use

AAV

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-YR-ICUE-mGold2t

Plasmid

#231790

Purpose

Visualization of cAMP dynamics using YR-ICUE-mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

YR-ICUE-mGold2t

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRZ-mOct4-CNL

Plasmid

#65718

Purpose

mOct4 reporter plasmid (expresses CNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Oct4 promoter and CNL

Use

Lentiviral and Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP_P2AT2A_GFP-nb-KS-FtoG

Plasmid

#238275

Purpose

For overexpression of TagBFP_P2AT2A_GFP-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP_P2AT2A_GFP-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP_P2AT2A_GFP-nb-KS

Plasmid

#238274

Purpose

For overexpression of TagBFP_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP_P2AT2A_GFP-nb

Plasmid

#238273

Purpose

For overexpression of TagBFP_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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