We narrowed to 23,494 results for: cel.2;

1. pGEX nsp1 K164A/H165A CoV2

   Plasmid

   #175514

   Purpose

   For protein expression of K164A/H165A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   K164A/H165A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX nsp1 R124A/K125A CoV2

   Plasmid

   #175513

   Purpose

   For protein expression of R124A/K125A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   R124A/K125A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX C_K nsp1 K164A/H165A CoV2

   Plasmid

   #175517

   Purpose

   Expresses C_K for bioconjugation on nsp1

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   C_K nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x

   Plasmid

   #182254

   Purpose

   Human PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoV2-Spike-D614G-Gamma

   Plasmid

   #177963

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)

   Depositor

   Melanie Ott

   Article

   Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

   Insert

   SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMV_CapChR2-GFP

   Plasmid

   #224357

   Purpose

   Expresses CapChR2-GFP in mammalian cells

   Depositor

   Adam Cohen

   Article

   Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

   Insert

   CapChR2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_ spike_del19

   Plasmid

   #155297

   Purpose

   Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

   Depositor

   Raffaele De Francesco

   Article

   Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

   Insert

   SARS-Cov2 spike_deleted (S SARS-Cov2)
   

   Use

   Generation of sars-cov-2-pseudotyped lentiviral p…

   Tags

   Expression

   Mutation

   Deletion in C-term 19 aa of spike

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pPPI4-Strep-SNAP-CD86-His10

   Plasmid

   #223570

   Purpose

   Protein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   CD86 (CD86 Human)
   

   Use

   Tags

   His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOX + nSlo2

   Plasmid

   #16202

   Depositor

   Larry Salkoff

   Article

   Yuan et al Nat Neurosci. 2000 Aug . 3(8):771-9.

   Insert

   Caenorhabditis elegans SLOwpoke potassium channel (slo-2 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pLV-tetO-Ets2

    Plasmid

    #70272

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Ets2 (Ets2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-tetO-Id2

    Plasmid

    #70764

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    inhibitor of DNA binding 2 (Id2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MTK7_011

    Plasmid

    #123878

    Purpose

    Encodes the 5' 500 bp hThal4.10-2-gh18 locus homology arm as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    RA_hTha5.10-2-hg18
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK234_047

    Plasmid

    #124055

    Purpose

    Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. spike-A192

    Plasmid

    #200887

    Purpose

    Expresses spike-A192 in E. coli bacterial cells

    Depositor

    Andrew MacKay

    Article

    Phan et al Biomacromolecules. 2023 Mar 31. doi: 10.

    Insert

    spike-A192 (S E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. 3XFlagNICD2

    Plasmid

    #20184

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 2 intracellular domain (Notch2 Mouse)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 2 Intracellular Domain (Met…

    Promoter

    CMV

    Available since

    Jan. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FLAG-SBP-STAG2 (WT)

    Plasmid

    #73963

    Purpose

    STAG2 cDNA (CCDS43990) wild type

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Use

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pKESaPar

    Plasmid

    #211829

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-ATXN1-30Q

    Plasmid

    #33238

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Smad2 (L+MH2)

    Plasmid

    #15730

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    Smad2 (L+MH2) (SMAD2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 187-467 (linker and MH2 regions).

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only