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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,416 results for: sequence

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Showing: 3481 - 3500 of 13416 results

  2. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

    Plasmid
    #108372
    Purpose
    Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108372-plasmid-map-sequence-id-209438

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  5. CFP(1-158)-RGS7t

    Plasmid
    #55761
    Purpose
    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    RGS7(202-477)-CFP(1-158) (RGS7 Human, Aequorea victoria)
    Tags
    CFP(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    The RGS sequence amino terminal to the GGL domain…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55761-plasmid-map-sequence-id-89566

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  9. pCDH-EF1-IRES eGFP

    Plasmid
    #128059
    Purpose
    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128059-plasmid-map-sequence-id-256675

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  10. pREP3xV5_ccdB

    Plasmid
    #79015
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79015-plasmid-map-sequence-id-151962

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  11. hTLR2 flag

    Plasmid
    #13082
    Depositor
    Ruslan Medzhitov
    Insert
    TLR2 (TLR2 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-16 have been replaced with mammalia…
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13082-plasmid-map-sequence-id-142444

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  12. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Expression
    Yeast
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  13. LentiGuide-10X-CS1-3'-puro

    Plasmid
    #212011
    Purpose
    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)
    Depositor
    Danwei Huangfu
    Insert
    gRNA scaffold
    Use
    Lentiviral
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212011-plasmid-map-sequence-id-419682

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  14. ORFtag_TetR-SD_Frame2

    Plasmid
    #220982
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220982-plasmid-map-sequence-id-437118

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  15. ORFtag_TetR-SD_Frame3

    Plasmid
    #220983
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
    Depositor
    Alexander Stark
    Insert
    TetR repressor
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220983-plasmid-map-sequence-id-437500

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  16. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  17. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Available Since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211448-plasmid-map-sequence-id-419419

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  18. pcDNA3-BG4-NLS-mVen

    Plasmid
    #211449
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Tags
    NLS-mVenus
    Expression
    Mammalian
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211449-plasmid-map-sequence-id-419262

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  19. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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  20. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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Showing: 3481 - 3500 of 13416 results