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Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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6-ISG-IFN
Plasmid#231488PurposeStandard for type I interferon signature (6 genes)DepositorInsertsUseQpcr plasmid standardMutationpartial sequenceAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Tc
Plasmid#222336PurposeExpresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3DepositorAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CRISPR_DpmrA
Plasmid#160903PurposeCRISPR_Cas9, lambda red recombineeringDepositorInsertsCas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette
PromoteraraBADAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-C1-PLCdelta-PH
Plasmid#21179Purposeencodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipidsDepositorInsertPLCdelta (PLCD1 Human)
TagsGFPExpressionMammalianMutationPH domain of human PLC delta, amino acids Met1–Il…Available SinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS1C
Plasmid#55168PurposeEmpty vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
ExpressionBacterialPromoterSmR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH124-ssr
Plasmid#198025PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Ap/Cb resistance cassette
ExpressionBacterialPromoterApR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH824-ssr
Plasmid#198027PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Apr resistance cassette
ExpressionBacterialPromoterAprR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
TagseGFPExpressionMammalianPromoterTRE3GAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
DDX5 WT V5
Plasmid#199576PurposeExpresses WT DDX5 cloned into pLenti puroDepositorAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateExpressionMammalianAvailable SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVP91A-pcaHG
Plasmid#113766PurposeExpresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.DepositorInsertsβ subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase
TagsMet-6xHisExpressionBacterialPromoterT5 promoterAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSP2-96-merTetO-EFS-BLaR
Plasmid#118713PurposeContains an optimized 96-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
ExpressionMammalianPromoterEFS-NSAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorExpressionMammalianPromoterCAGGSAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 T204D
Plasmid#80877Purposemammalian expression of constitutively active ALK5 T204DDepositorInsertALK5 (TGFBR1 Human)
TagsHAExpressionMammalianMutationT204D (constitutively active)PromoterCMVAvailable SinceAug. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP2-48-merTetO-EFS-BLaR
Plasmid#118712PurposeContains an optimized 48-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
ExpressionMammalianPromoterEFS-NSAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIBR-Int4-Cas9-NT
Plasmid#235166PurposeExpresses the SIBR-Int4-Cas9 system and a NT sgRNADepositorInsertSIBR-Int4-Cas9
ExpressionBacterialAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
ins:CFP-NTR
Plasmid#66593PurposeSpecific and conditional cell ablation of beta cells in the zebrafish pancreasDepositorInsertInsulin promotor driving CFP-nitroreductase
UseZebrafish expressionTagsCFP fused with N-terminus of nitroreductasePromoterZebrafish Insulin promoter (approximately 1.2kb)Available SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only