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double_sgRNA targeting e1 and e4

Plasmid

#190688

Purpose

sgRNAs targeting enhancer 1 and 4 of MYC separately

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 1 and 4 of MYC separately

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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L1-AA027

Plasmid

#185752

Purpose

Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

HygR2

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2341_Tier3(SB)-Puro

Plasmid

#169639

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2403-Tier1-PhCMV-ZeoR

Plasmid

#169495

Purpose

Tier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Sh ble

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_5

Plasmid

#172470

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_6

Plasmid

#172471

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1.

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Pb mD3S4-dN

Plasmid

#180252

Purpose

P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

Depositor

Timothy Springer

Article

Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

Insert

D3 of P. berghei HAP2 with N-glycosylation sites mutated

Tags

6His

Expression

Mammalian

Mutation

N516T, S533N and N539Q

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B S395E

Plasmid

#176006

Purpose

Expresses WIPI2B (S395E) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (S395E) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Serine 395 to Glutamate

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B S395A

Plasmid

#176005

Purpose

Expresses WIPI2B (S395A) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (S395A) (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Serine 395 to Alanine

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJPS2952

Plasmid

#135586

Purpose

SNR6 has UUC at position 105–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ3 has UUC at position 105–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2953

Plasmid

#135587

Purpose

SNR6 has AUUUC at position 103–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ5 has AUUUC at position 103–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2955

Plasmid

#135589

Purpose

SNR6 has UUUAUUUC at position 100–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ8 has UUUAUUUC at position 100–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pZA17

Plasmid

#158495

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p415:iCas9

Plasmid

#138266

Purpose

Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mTN3-GGS6-dCas9

Use

CRISPR

Expression

Yeast

Mutation

Cas9-D10A, H840A

Promoter

GalL

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hsg(NT:NT)

Plasmid

#138260

Purpose

Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-FeGFP

Plasmid

#138261

Purpose

Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

eGFP

Use

CRISPR

Expression

Mammalian

Promoter

None

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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UL79(E48A_L49A)-3xFLAG pCDNA4

Plasmid

#129759

Purpose

Expresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL79 (UL79 Human Cytomegalovirus (HHV-5))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glutamic acid 48 to Alanine and Leucine 4…

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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