We narrowed to 21,037 results for: cas9 genes

1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide2

   Plasmid

   #118201

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs7903146-guide3

   Plasmid

   #118200

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide4

   Plasmid

   #118203

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs11257655-guide3

   Plasmid

   #118202

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

   Plasmid

   #107301

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
   

   Use

   CRISPR

   Tags

   FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

   Plasmid

   #101181

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

    Plasmid

    #101182

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid

    #101183

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid

    #101180

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/Q926A

    Promoter

    CMV

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

    Plasmid

    #69231

    Purpose

    Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 TALE
    

    Use

    CRISPR

    Tags

    2X NLS and NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSET-dCas9-actII-4-NT-S1

    Plasmid

    #110185

    Purpose

    Repression of gene expression in Streptomyces by CRISPRi

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Inserts

    dCas9
    sgRNA
    

    Use

    CRISPR; I

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    J23119 and ermE*p

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTrex-b-NLS-hSpCas9

    Plasmid

    #62543

    Purpose

    Expression of Cas9 in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    NLS-hSpCas9
    

    Use

    CRISPR; Trypanosoma cruzi expression

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Ribo-HX1

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG Cas9-2A-Citrine

    Plasmid

    #92358

    Purpose

    CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSAG1::CAS9-U6::sgUPRT

    Plasmid

    #54467

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

    Depositor

    David Sibley

    Article

    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

    Insert

    SAG1 5' UTR
    

    Use

    CRISPR; Toxoplasma expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-idCas9-vpr

    Plasmid

    #89985

    Purpose

    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

    Depositor

    Jie Na

    Article

    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

    Insert

    dCas9-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge