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Showing: 5251 - 5280 of 8427 results

  1. pST50Trc1-DHFRFLAG

    Plasmid
    #63951
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63951-plasmid-map-sequence-id-177357

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  3. pCDW115

    Plasmid
    #70062
    Purpose
    artificial transcription template "pause ablation"
    Depositor
    Jeff Gelles
    Article
    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi
    Insert
    lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pST50Trc4-DHFRHIS

    Plasmid
    #64003
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    64003-plasmid-map-sequence-id-177409

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  5. pST50Trc4-DHFRFLAG

    Plasmid
    #64006
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64006-plasmid-map-sequence-id-177412

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  11. pST50Trc3-DHFRHIS

    Plasmid
    #63985
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    63985-plasmid-map-sequence-id-177391

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  12. pZNF416-donor

    Plasmid
    #112372
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF416 with GFP
    Depositor
    Kevin White
    Insert
    ZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:57572882:A>G,hg38:19:57572932:C>G,h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112372-plasmid-map-sequence-id-229731

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  16. pST50Trc3-DHFRFLAG

    Plasmid
    #63987
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    63987-plasmid-map-sequence-id-177393

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  17. pST50Trc4-DHFRCYD

    Plasmid
    #64005
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    CYD
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64005-plasmid-map-sequence-id-177411

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  18. pST50Trc2-DHFRFLAG

    Plasmid
    #63969
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63969-plasmid-map-sequence-id-177375

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  19. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233046
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233046-plasmid-map-sequence-id-467426

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  20. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233045
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233045-plasmid-map-sequence-id-467425

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  21. psLIB U6

    Plasmid
    #229956
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229956-plasmid-map-sequence-id-461573

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  22. AA498

    Plasmid
    #231119
    Purpose
    Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control
    Depositor
    John Doench, David Root
    Insert
    sgCD274 + ABE splice-targeting sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231119-plasmid-map-sequence-id-458365

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  23. AA505

    Plasmid
    #231120
    Purpose
    Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control
    Depositor
    John Doench, David Root
    Insert
    sgCD274 + CBE splice-targeting sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231120-plasmid-map-sequence-id-458364

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  24. tRECs/3xUAS-FP attB

    Plasmid
    #220330
    Purpose
    encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)
    Depositor
    Erdem Bangi
    Inserts
    B3RT-STOP-B3RT-TagBFP-3xHA
    KDRT-STOP-KDRT-GFP-HSV
    FRTm71-STOP-FRTm71-mCherry
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    220330-plasmid-map-sequence-id-441802

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  26. pBH502

    Plasmid
    #215781
    Purpose
    NBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting Nanoluc
    Depositor
    Corey Wilson
    Article
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    Nanoluc
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bt1311, P(O1), and cfxA
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215781-plasmid-map-sequence-id-430541

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Showing: 5251 - 5280 of 8427 results