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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,939 results for: 11

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Showing: 3501 - 3520 of 7939 results

  1. DT12A

    Plasmid
    #80416
    Purpose
    Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80416-plasmid-map-sequence-id-457373

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  3. pCAG-GFP-PQRv3-RFP

    Plasmid
    #73951
    Purpose
    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.
    Depositor
    Brian Chen
    Article
    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do
    Inserts
    sfGFP (superfolder GFP)
    RFP
    Tags
    A residual PQR peptide wii be left at the C-termi…
    Expression
    Mammalian
    Promoter
    Chicken beta actin and Chicken beta actin (shared…
    Available Since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. DT40

    Plasmid
    #80415
    Purpose
    Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80415-plasmid-map-sequence-id-151402

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  17. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  18. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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  20. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Promoter
    cmv
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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Showing: 3501 - 3520 of 7939 results