We narrowed to 3,645 results for: MAK;

1. mCerulean3-Mcl-1-pLVX

   Plasmid

   #166752

   Purpose

   Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, Mcl-1, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Mcl-1 (MCL1 Human)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-I-Sce1-eno2:Tau-IRES-egfp

   Plasmid

   #112205

   Purpose

   Used to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoter

   Depositor

   Edward Burton

   Article

   Bai et al Nucleic Acids Res. 2007;35(19):6501-16.

   Insert

   MAPT (MAPT Human)
   

   Use

   Zebrafish expression

   Tags

   eno2 exon 2 translational fusion, ires-egfp at c …

   Expression

   Mutation

   Promoter

   eno2 from zebrafish

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIN- myc ULK1 K46I

   Plasmid

   #27627

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2010 Dec 23. ():.

   Insert

   mouse myc ULK1 K46I (Myc Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mutation

   Initial M from ULK1 removed. Silent mutation at A…

   Promoter

   Available since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-RepoMan-wt

   Plasmid

   #184269

   Purpose

   Expresses shRNA resistant GFP-RepoMan

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   changed nucleotides 723-747, 1488-1509, 1524-1548…

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-BCL-XL-499R

   Plasmid

   #203574

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5- tagged

   Expression

   Mammalian

   Mutation

   silent point mutations introduced to make it resi…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-SEMA3E-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156970

   Purpose

   Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3E (SEMA3E Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28B-PU.1-ETS-Wing

   Plasmid

   #124552

   Purpose

   Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

   Depositor

   Gregory Poon

   Article

   Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

   Insert

   PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   The 5 residues making up the "wing" in …

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO/Strep-HA-ZAK alpha

   Plasmid

   #141193

   Purpose

   Expresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 (MAP3K20 Human)
   

   Use

   Tags

   Strep, HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIN-PAmCherry-mSNCA-NE

   Plasmid

   #102364

   Purpose

   Lentiviral overexpression of PA-mCherry synuclein alpha fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   synuclein alpha (Snca Mouse)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pinducer 20 DN-KASHΔPPPL

    Plasmid

    #129280

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

    Depositor

    Daniel Conway

    Article

    Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    the last 4 amino acids (PPPL) of the KASH domain …

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/Strep-HA-ZAK beta

    Plasmid

    #141195

    Purpose

    Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 isoform 2 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIN-hSNCA-NE

    Plasmid

    #102366

    Purpose

    Lentiviral overexpression of synuclein alpha

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Insert

    synuclein alpha (SNCA Human)
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    A silent mutation (333bp downstream of ATG) was i…

    Promoter

    EF-1a

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO/GFP-GIGYF2

    Plasmid

    #141189

    Purpose

    Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    GIGYF2 (GIGYF2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO/Strep-HA-ZAKalpha_deltaCTD

    Plasmid

    #141196

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

    Plasmid

    #141194

    Purpose

    Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Changed Lysine 45 to Alanine

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid

    #109430

    Purpose

    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid

    #102421

    Purpose

    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Modified at the Dharmacon SMARTpool siRNA #2 targ…

    Promoter

    TetO-CMV

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid

    #102422

    Purpose

    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

    Depositor

    Kian Peng Koh

    Article

    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

    Insert

    Tet1 catalytic domain mutant (Tet1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1620Y and D1622A mutations in Tet1 catalytic dom…

    Promoter

    TetO-CMV

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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