We narrowed to 26,884 results for: STI

1. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RMCE K>R DNA-PKcs

   Plasmid

   #233917

   Purpose

   Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K3753R

   Promoter

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

   Plasmid

   #102421

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Modified at the Dharmacon SMARTpool siRNA #2 targ…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

   Plasmid

   #208959

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-MPSV-eBFP2

   Plasmid

   #220472

   Purpose

   Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

   Depositor

   Christian Krebs

   Article

   Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

   Plasmid

   #208958

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209060

   Purpose

   Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

   Insert

   sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
   

   Use

   Gateway vector to be used for lr reaction

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

   Plasmid

   #217800

   Purpose

   Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

    Plasmid

    #217805

    Purpose

    Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-linker-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-hDAGLa-V5

    Plasmid

    #87674

    Purpose

    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Article

    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do

    Insert

    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    

    Use

    Tags

    HA and V5 tag

    Expression

    Mammalian

    Mutation

    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

    Promoter

    T7

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX307-mFzd4

    Plasmid

    #102866

    Purpose

    Expresses mouse Fzd4 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T to C substitution at nucleotide 1149 of Fzd4 OR…

    Promoter

    EF1A

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

    Plasmid

    #208039

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa; 3MAS) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Expression

    Mutation

    3MAS mutant: K65R, K160R and K490R; mutated SIM

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-BLAST flag-CAD hCPS1 AD

    Plasmid

    #188130

    Purpose

    Expresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307-mFzd9

    Plasmid

    #102877

    Purpose

    Expresses mouse Fzd9 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Frizzled 9 (Fzd9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A to G substitution at nucleotide 114, G to A sub…

    Promoter

    EF1A

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-gamma2S/deltaIL

    Plasmid

    #119730

    Purpose

    GABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Article

    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

    Insert

    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MB 94C thsS(t3)R-Bxb1_P7-sfGFP_mCherry

    Plasmid

    #232471

    Purpose

    Optimized thiosulfate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry

    Depositor

    Mikhail Shapiro

    Article

    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

    Inserts

    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Bxb1 integrase
    

    Use

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Contains the following mutations K286Q, Q350H, I…

    Promoter

    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CROPseq-SFFV-eBFP2

    Plasmid

    #239601

    Purpose

    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

    Depositor

    Christian Krebs

    Article

    Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

    Insert

    spleen focus‑forming virus - promotor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid

    #217803

    Purpose

    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)

    Plasmid

    #217807

    Purpose

    Unfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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