We narrowed to 23,576 results for: CHI

1. p2T-CMV-AIDmax-BlastR

   Plasmid

   #152994

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   AID-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2T-CMV-ABEmaxCP1041-BlastR

   Plasmid

   #152990

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   ABE7.10-nSpCas9 CP1041
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAJM.847

   Plasmid

   #108524

   Purpose

   PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PhlFAM + PPhlF-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p2T-CMV-BE4maxCP1028-BlastR

   Plasmid

   #152993

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9 CP1028-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSW068

   Plasmid

   #140036

   Purpose

   KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Truncated thioesterase gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cLac143

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Smac(1-60)mCherry

   Plasmid

   #40880

   Depositor

   Douglas Green

   Article

   Tait et al Dev Cell. 2010 May 18;18(5):802-13.

   Insert

   Smac(1-60)mCherry (DIABLO Human)
   

   Use

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pUE1-TSP-BFLF1

   Plasmid

   #162657

   Purpose

   Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   BFLF1
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDual-eGFP

   Plasmid

   #63215

   Purpose

   Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV-EF1α hybrid (CEF)

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    p-T5 Lac

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB700 lenti gRNA Blasto_zhang2.0

    Plasmid

    #167912

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Nano-lantern(cAMP-1.6)/pRSETB

    Plasmid

    #53591

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

13. sgTrack-mCherry

    Plasmid

    #114013

    Purpose

    Empty sgRNA expression vector with co-expression of mCherry reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120395

    Purpose

    pAAV plasmid expressing scrambled shRNA under the U6 promoter and mCherry.3xFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Scrambled shRNA
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

    Plasmid

    #75203

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p2T-CMV-CDAmax-BlastR

    Plasmid

    #152995

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    CDA-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pScPPX2

    Plasmid

    #112877

    Purpose

    Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

    Depositor

    Michael Gray, Ursula Jakob

    Article

    Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

    Insert

    PPX1 (PPX1 Budding Yeast)
    

    Use

    Tags

    6X His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgTrack-GFP

    Plasmid

    #114012

    Purpose

    Empty sgRNA expression vector with co-expression of TurboGFP reporter

    Depositor

    Charles Rudin

    Article

    Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Use

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pShuttle-FEN1hWT

    Plasmid

    #35027

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1 (FEN1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAG-FLEx(FRT)-TC

    Plasmid

    #67827

    Purpose

    Flp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cells

    Depositor

    Liqun Luo

    Article

    Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

    Insert

    TVA-mCherry fusion
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti_CMV: Flag-Antares_SV40:PuroR

    Plasmid

    #183049

    Purpose

    Expresses FLAG-tagged Antares in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    Antares BRET reporter
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDisplay-NE2m-IRES-mCherry-CAAX

    Plasmid

    #208690

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AsCas12a

    Plasmid

    #188492

    Purpose

    Expresses FLAG tagged AsCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmiRFP720-tubulin

    Plasmid

    #136574

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP720-tubulin
    

    Use

    Tags

    miRFP720

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEM705 Gst (Ser)

    Plasmid

    #109354

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAJT195

    Plasmid

    #202294

    Purpose

    Type IIS cloning plasmid for LPattB7 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPP381

    Plasmid

    #158798

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RuvC-inactivating mutation D394A

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET15b T7pCONS TIR-2 sfGFP

    Plasmid

    #154468

    Purpose

    pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAJM.661

    Plasmid

    #108532

    Purpose

    TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TtgRAM + PTtg-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TtgR: R130H A144T A148V

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWZL-Blast-Flag-HA-hTERT

    Plasmid

    #22396

    Depositor

    William Hahn

    Article

    Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

    Insert

    TERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBbS8c-ddcpf1-Δ

    Plasmid

    #153039

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLG021 (AVAST type 5)

    Plasmid

    #157899

    Purpose

    Expresses the AVAST type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 5 (STAND ATPase with N-terminal SIR2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2-Mito-mRFP

    Plasmid

    #169001

    Purpose

    Express Mito (Su9)-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMUT2-pTcpP-mCherry

    Plasmid

    #192861

    Purpose

    pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBBK

    Plasmid

    #62670

    Purpose

    Vector for PhiC31o mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MiniCoopR 2xU6:gRNA, mitfa:Cas9

    Plasmid

    #118844

    Purpose

    Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLOX-CW-CRE

    Plasmid

    #12238

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    CRE
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Shp2 biosensor

    Plasmid

    #134346

    Purpose

    FRET biosensor. To visualize Shp2 activity by FRET

    Depositor

    Yingxiao Wang

    Article

    Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

    Insert

    Shp2 Biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDe-CAS9-D10A

    Plasmid

    #61477

    Purpose

    based on pDe-CAS9, encodes Cas9 D10A nickase variant

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    catalytic Asp10 changed to Ala

    Promoter

    PcUbi4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSB700 lenti gRNA EYFP

    Plasmid

    #167906

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCS2-LAMP1-mRFP

    Plasmid

    #169004

    Purpose

    Express rat LAMP1-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

    Insert

    rat LAMP1-mRFP
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAJM.1642

    Plasmid

    #108535

    Purpose

    CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    CinRAM + PCin-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    CinR: S72C V80I G114D G115D R137H K220R A224T *24…

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pTrc-trGPPS(CO)-LS

    Plasmid

    #50603

    Purpose

    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

    Depositor

    Jay Keasling

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Inserts

    Limonene Synthase
    ApR
    LacIq
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    truncated codon optimized geranylpyrophosphate sy…

    Promoter

    Trc

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAJM.690

    Plasmid

    #108533

    Purpose

    PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PcaUAM + P3B5B-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PcaU: K14N A165T N201H

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Yellow Cameleon-Nano15/pcDNA3

    Plasmid

    #51961

    Purpose

    mammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only