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We narrowed to 6,562 results for: nls

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Showing: 3521 - 3540 of 6562 results

  2. pMSCVpuro-F-Gcn5-NLSm

    Plasmid
    #63708
    Purpose
    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells
    Depositor
    Kai Ge
    Publication
    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:
    Insert
    Gcn5 (Kat2a Mouse)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    mutated K425, K427 and K438 to R (arginine)
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. mKO2-PCNA-19-SV40NLS-4

    Plasmid
    #57890
    Purpose
    Localization: Nucleus, Excitation: 551, Emission: 565
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mKO2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    57890-plasmid-map-sequence-id-233994

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  7. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  10. pAAV_BiLAMP5e3_dTomato_nlsdTomato (AAV PHP.eB)

    Viral prep
    #218792-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiLAMP5e3_dTomato_nlsdTomato (#218792). In addition to the viral particles, you will also receive purified pAAV_BiLAMP5e3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the Lamp5 interneuron-targeting enhancer. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Available since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV_BiCHATe27_dTomato_nlsdTomato (AAV PHP.eB)

    Viral prep
    #213829-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_dTomato_nlsdTomato (#213829). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    dTomato
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only

  12. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Publication
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    221002-plasmid-map-sequence-id-436162

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  14. pY31

    Plasmid
    #84746
    Purpose
    Expresses huLbCpf1-P2A-puro and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huLbCpf1
    puromycin resistance gene
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84746-plasmid-map-sequence-id-162433

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  15. pAAV_BiCHATe27_dTomato_nlsdTomato (AAV1)

    Viral prep
    #213829-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_BiCHATe27_dTomato_nlsdTomato (#213829). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the cholinergic neuron-targeting enhancer E27. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Tags
    dTomato
    Available since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  17. M-SPn-VP64

    Plasmid
    #48674
    Purpose
    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null
    Promoter
    CMV
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles

  18. SID4x-dCas9-KRAB

    Plasmid
    #106399
    Purpose
    Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain
    Depositor
    Jason Gertz
    Publication
    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SID4x (MXD1 Human)
    KRAB (ZNF10 Human)
    Tags
    FLAG, HA, and NLS
    Expression
    Mammalian
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    106399-plasmid-map-sequence-id-207594

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  19. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    138162-plasmid-map-sequence-id-272344

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Showing: 3521 - 3540 of 6562 results