We narrowed to 6,241 results for: nls

1. pb-CAGGS-dCas9-VP64-blasto

   Plasmid

   #218556

   Purpose

   Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance

   Depositor

   Alejandro Chavez

   Insert

   dCas9
   

   Use

   CRISPR; Piggybac transposition

   Tags

   2xSV40NLS-VP64

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

   Plasmid

   #218558

   Purpose

   Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

   Depositor

   Alejandro Chavez

   Insert

   MCP-MLLx3-HSF1
   

   Use

   Lentiviral

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

   Plasmid

   #218559

   Purpose

   Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

   Depositor

   Alejandro Chavez

   Insert

   MCP-P65-HSF1
   

   Use

   Lentiviral

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195623

   Purpose

   Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   EF1a (Mlu1/EcoR1)

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-MP-84-spCas9-U6-gRNA-tlock

   Plasmid

   #206936

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   MP-84

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW121-SS-281

   Plasmid

   #68769

   Purpose

   CIS-V3m with N-FLAG and C-HIS

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   CIS-V3m
   

   Tags

   HIS, NLS, and flag

   Expression

   Mammalian

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW121-SS-279

   Plasmid

   #68767

   Purpose

   CIS-V2m with N-FLAG and C-HIS

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   CIS-V2m
   

   Tags

   HIS, NLS, and flag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW121-SS-280

   Plasmid

   #68768

   Purpose

   CIS-V3 with N-FLAG and C-HIS

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Insert

   CIS-V3
   

   Tags

   HIS, NLS, and flag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-EF1a-BsdCas9-W

   Plasmid

   #67978

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-EF1a-Cas9Bsd-W

    Plasmid

    #68343

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid

    #75140

    Purpose

    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 91-710

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Icam 2 SaCas9 YAP sgRNA1

    Plasmid

    #99734

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid

    #223136

    Purpose

    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized PEmax-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

    Expression

    Mammalian

    Mutation

    M-MLV mutations from PE2 (Anzalone et al. Nature …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Icam 2 SaCas9 YAP sgRNA2

    Plasmid

    #99735

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Icam 2 SaCas9 YAP sgRNA3

    Plasmid

    #99736

    Purpose

    Expresses SaCas9 and a gRNA targeting mouse YAP

    Depositor

    Yu Huang

    Article

    Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

    Insert

    SaCas9 gRNA targeting Yap1 (Yap1 Mouse)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHygG2

    Plasmid

    #200066

    Purpose

    hygR selection nls-mNG marked rapid RMCE integration vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

    Type

    Empty backbone

    Use

    Recombination mediated cassette exchange

    Expression

    Worm

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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