We narrowed to 7,306 results for: GFP expression plasmids

1. pMR506-EF1a-H2B-EGFP

   Plasmid

   #186627

   Purpose

   Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

   Depositor

   Jonas Frisén

   Article

   Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

   Insert

   EF1a (EEF1A1 Synthetic)
   

   Use

   Lentiviral

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCherryGFP-CoV2-Inframe-stop

   Plasmid

   #177620

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac3-EcTrp-TGA-EGFP**

   Plasmid

   #228786

   Purpose

   EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   EGFP**
   

   Use

   Baculoviral

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

   Plasmid

   #220797

   Purpose

   shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

   Insert

   EGFP
   

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS34.EF1α<GFP-2A-Cas9-RC

   Plasmid

   #207404

   Purpose

   Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Cas9-RC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP-2A

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. attB-GFP-Dnmt3b1-Poly(A)

   Plasmid

   #65531

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 1

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-H1-shSRGAP2A-hsynapsin-EGFP

   Plasmid

   #220798

   Purpose

   shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

   Depositor

   Pierre Vanderhaeghen

   Article

   Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

   Insert

   EGFP
   

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACT1:Cas9-GFP, U6:sgTK

   Plasmid

   #122852

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgTK
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACT1:Cas9-GFP, U6:sgUPRT

   Plasmid

   #122853

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgUPRT
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSAD-5PSD95-EGFP-SynPhRFP

    Plasmid

    #217979

    Purpose

    G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

    Depositor

    Edward Callaway

    Article

    Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

    Inserts

    5PSD95-EGFP (Dlg4 Rat)
    SynPhRFP (Syp Mouse)
    

    Use

    G-deleted rabies

    Tags

    EGFP and RFP

    Promoter

    None

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2/GD-IRES-GFP-CTNNB1

    Plasmid

    #192868

    Purpose

    Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

    Depositor

    David Largaespada

    Article

    Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

    Inserts

    luc+
    EGFP
    CTNNB1
    

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. attB-GFP-Tet1-Poly(A)

    Plasmid

    #65559

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB-GFP-Dnmt3b6-Poly(A)

    Plasmid

    #65532

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-shCasp2-IRES-GFP

    Plasmid

    #52061

    Purpose

    stable knockdown of Caspase-2 in mammalian cells

    Depositor

    Trudy Oliver, Tyler Jacks

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Caspase-2 shRNA (CASP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

    Plasmid

    #85821

    Purpose

    iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    Zf Ubi::cMyc oDam_f_GFPoNLS
    

    Use

    Tol2 based vector

    Tags

    cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-H1-shSRGAP2B/C#1-hsynapsin-EGFP

    Plasmid

    #220796

    Purpose

    shRNA plasmid against human SRGAP2B/C genes (#1) with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEos2-dVHH[GFP] in pCSDEST

    Plasmid

    #162870

    Purpose

    Super-resolution of GFP in culture

    Depositor

    Rob Parton

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    mEos2-dVHH[EGFP]
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid

    #140001

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-UBC-HLA-E SCT-T2A-GFP

    Plasmid

    #205461

    Purpose

    lentiviral plasmid for expression of HLA-E SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-E SCT (HLA-E Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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