We narrowed to 30,758 results for: LIS

1. pBabe-Flag-SKP1-T131A

   Plasmid

   #213734

   Purpose

   Expresses human SKP1 T131A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-T131A (SKP1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST17-ALDH1A3

   Plasmid

   #189750

   Purpose

   The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX317-MBNL2

   Plasmid

   #115444

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   MBNL2 (MBNL2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216249

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgRANGAP.C1 (RANGAP1 Human)
   

   Use

   CRISPR; Endogenous tagging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-pegRNA-HEK3-CTTins-px330-scaffold

   Plasmid

   #180017

   Purpose

   Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGIPz-mPD-L1 3SA

   Plasmid

   #121489

   Purpose

   Expression of mouse PD-L1 3SA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1 3SA
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S175A, T179A, S183A

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-pegRNA-LRRK2-G2019S-3a

   Plasmid

   #180432

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1-shMBNL1-9155

   Plasmid

   #115460

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shMBNL1-9155 (MBNL1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-PGK-EGFP-ALDH1A3-Neo

   Plasmid

   #189744

   Purpose

   The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST17-ALDH1A1

    Plasmid

    #189749

    Purpose

    The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A1 (ALDH1A1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGIPz-PD-L1 3SA

    Plasmid

    #121490

    Purpose

    Expression of human PD-L1 3SA with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Inserts

    PD-L1 3SA
    humun PD-L1 shRNA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S176A, T180A, S184A

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO.1-shMBNL2-3712

    Plasmid

    #115461

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL2-3712 (MBNL2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1-shMBNL2-1082

    Plasmid

    #115462

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL2-1082 (MBNL2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-shMBNL1-3964

    Plasmid

    #115459

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL1-3964 (MBNL1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-PD-L1 3SA/4NQ

    Plasmid

    #121491

    Purpose

    Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1-shMBNL3-0455

    Plasmid

    #115463

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL3-0455 (MBNL3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-shMBNL3-9465

    Plasmid

    #115464

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shMBNL3-9465 (MBNL3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dCas9-NONO2_IDR-EGFP

    Plasmid

    #232765

    Purpose

    Expresses dCas9-NONO2_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NONO2_IDR-EGFP (NONO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dCas9-NUP98b_IDR-EGFP

    Plasmid

    #232767

    Purpose

    Expresses dCas9-NUP98b_IDR-EGFP

    Depositor

    Isaac Hilton

    Article

    Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

    Insert

    dCas9-NUP98b_IDR-EGFP (NUP98 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge