We narrowed to 15,157 results for: pET

1. pETDUET-GST AO7RE I137A

   Plasmid

   #139319

   Purpose

   GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed isoleucine 137 to an alanine

   Promoter

   T7 promoter-1

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Himar-dCas9-mammalian

   Plasmid

   #138414

   Purpose

   Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Tags

   3xFLAG, 6xHis, and SV40 NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

   Plasmid

   #136688

   Purpose

   Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 (BECN1 Human)
   

   Tags

   Trigger Factor

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression w/ three mut…

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b-3xVSVG-GSK3(1-97)

   Plasmid

   #129583

   Purpose

   Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Insert

   GSK3a(1-97) (GSK3A Human)
   

   Tags

   6xHistidine

   Expression

   Bacterial

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJUMP18-RBS-pET-MV_RN

   Plasmid

   #127014

   Purpose

   Basic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part RBS-pET-MV_RN
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-VHH-tev-2xTS

   Plasmid

   #117750

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFS_0362_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._BA-91_ARRAY_1

   Plasmid

   #116974

   Purpose

   Expresses CaBA-91RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Candidatus Accumulibacter sp. BA-91 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFS_0356_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1_RC

   Plasmid

   #116968

   Purpose

   Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFS_0355_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1

   Plasmid

   #116967

   Purpose

   Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b-clbH-C-A2-PCP S1524A

    Plasmid

    #114158

    Purpose

    Expresses ClbH-C-A2-PCP S1524A

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbH
    

    Tags

    S-Tag, Thrombin site

    Expression

    Bacterial

    Mutation

    Only contains the C-A2-PCP domains of ClbH with S…

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-30a(+)-Syk-tSH2-FX

    Plasmid

    #111272

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
    

    Expression

    Bacterial

    Mutation

    interdomain A (Phe 119 to His 162) substituted by…

    Promoter

    T7 promoter

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-ecDHFR-dCys-T46C

    Plasmid

    #110543

    Purpose

    Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    T46 mutated to C46, C85 mutated to A85, C152 muta…

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b-ecDHFR-dCys-L54C

    Plasmid

    #110542

    Purpose

    Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    L54 mutated to C54, C85 mutated to A85, C152 muta…

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-ecDHFR-D27S/Y100F

    Plasmid

    #110827

    Purpose

    Bacterial expression of E.coli DHFR D27S/Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Mutation

    D27 mutated to S27, Y100 mutated to F100

    Promoter

    T7

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-NmCas9-Flag D16A

    Plasmid

    #109031

    Purpose

    D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Tags

    Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETDuet RagA(R37P)-RagC

    Plasmid

    #99646

    Purpose

    Bacterial expression of RagA(R37P)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    R37P

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet RagA-RagC(K84T))

    Plasmid

    #99648

    Purpose

    Bacterial expression of RagA-RagC(K84T)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    K84T

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pet151D-TOPO-BROX 1-401

    Plasmid

    #89866

    Purpose

    Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    expresses only residues 1-401

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. csd2_121-308,S166A/pET28b(+)

    Plasmid

    #83301

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag, mutation: S166A

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120, and changed Serine 166 …

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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