We narrowed to 2,328 results for: dap

1. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213028

   Purpose

   Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   E43S D145N double mutant EN- endonuclease catalyt…

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213027

   Purpose

   Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   D702Y (RT- reverse transcriptase catalytic dead)

   Promoter

   CMV

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

6. T-B18

   Bacterial Strain

   #159446

   Purpose

   E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon

   Depositor

   Eleftherios Terry Papoutsakis, Maciek R. Antoniewicz

   Article

   Har et al Appl Microbiol Biotechnol. 2021 Jan;105(

   Bacterial Resistance

   Ampicillin and kanamycin

   Available since

   Aug. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

7. Human Kinase Domain Constructs for Automated Bacterial Expression

   Plasmid Kit

   #1000000094

   Purpose

   68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBP-P_phlF-PPhlF

   Plasmid

   #204075

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   phlF repressor and PPhlF DAPG-inducible promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

   Plasmid

   #204080

   Purpose

   Type 0 promoter part

   Depositor

   Mikhail Shapiro

   Insert

   TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mr-GST full length

    Plasmid

    #112982

    Purpose

    For protein expression and purification of full-length Drosophila mr (APC2)

    Depositor

    Terry Orr-Weaver

    Insert

    mr (APC2) (mr Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only