We narrowed to 75,518 results for: LAS

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pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-His-SUMO1

Plasmid

#17271

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

SUMO1 (SUMO1 Human)

Tags

6xHis

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
Hsp68-LacZ-Gateway

Plasmid

#37843

Depositor

Nadav Ahituv

Article

Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

Type

Empty backbone

Use

Mouse Targeting

Promoter

Mouse Hsp68 minimal promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-mCherry

Plasmid

#220353

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

Plasmid

#35511

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

Plasmid

#156445

Purpose

For transient expression of mouse RAD21 (SCC1) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse RAD21 (Rad21 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

Plasmid

#156436

Purpose

For transient expression of human PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5B (PDS5B Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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hWIP1 D314A FLAG

Plasmid

#28106

Depositor

Lawrence Donehower

Article

Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

Insert

WIP1 (PPM1D Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains phosphatase-dead mutation D314A

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
VP16-ER alpha

Plasmid

#11351

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER alpha (ESR1 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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NET5L pEGFP-N2 (1155)

Plasmid

#62035

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

NET5L (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

long form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
TJP1_PDZ_2

Plasmid

#103910

Purpose

Protein expression and purification of ZO1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD_sfGFP3TCG4TAG

Plasmid

#174518

Purpose

Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

sfGFP

Tags

6 x His

Expression

Bacterial

Mutation

Recoded. TCG and TAG codons were introduced in po…

Promoter

araBAD

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_1

Plasmid

#103909

Purpose

Protein expression and purification of ZO1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-GFP

Plasmid

#220350

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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gCH27 (crCD55-4)

Plasmid

#217343

Purpose

crRNA targeting CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD55-4 (CD55 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pdCas9::BFP-humanized

Plasmid

#44247

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 fused to BFP

Use

CRISPR and Retroviral

Tags

2xNLS & tagBFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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gCH29 (crCD81-1)

Plasmid

#217344

Purpose

crRNA targeting CD81

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crCD81-1 (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-Renilla cDNA

Plasmid

#29783

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

Renilla

Use

Lentiviral

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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Ad-EphA3 KD

Plasmid

#102726

Purpose

Expression of Kinase dead EphA3

Depositor

Jin Chen

Article

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Mutation

Kinase Dead (K657M; AAA > ATG)

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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