Skip to main content
Addgene
Showing: 511 - 540 of 1225 results

  1. pTS2

    Plasmid
    #87798
    Purpose
    For constructing SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87798-plasmid-map-sequence-id-177271

    Enlarge

  2. pTMS2

    Plasmid
    #87800
    Purpose
    For constructing MBP-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87800-plasmid-map-sequence-id-177268

    Enlarge

  3. pTNS1

    Plasmid
    #87801
    Purpose
    For constructing NusA-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87801-plasmid-map-sequence-id-177272

    Enlarge

  8. MZB211

    Plasmid
    #228494
    Purpose
    10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)
    Depositor
    Richard Baker
    Insert
    pol
    Use
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Mutation
    A408S
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228494-plasmid-map-sequence-id-455979

    Enlarge

  9. MZB174

    Plasmid
    #228487
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)-GST-HRV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228487-plasmid-map-sequence-id-455394

    Enlarge

  10. MZB205

    Plasmid
    #228490
    Purpose
    Empty 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Use
    Tags
    10xHis-SUMO(Smt3)-Avitag-HRV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228490-plasmid-map-sequence-id-455387

    Enlarge

  13. MZB139

    Plasmid
    #217838
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    SMT3 (SMT3 Budding Yeast)
    Use
    Tags
    10x His and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217838-plasmid-map-sequence-id-432993

    Enlarge

  18. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

    Enlarge

  25. 371-S

    Plasmid
    #97034
    Purpose
    His6-Sumo-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, SUMO domain, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97034-plasmid-map-sequence-id-182498

    Enlarge

  27. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

    Plasmid
    #208039
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    PML (isoform IVa; 3MAS) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Expression
    Mutation
    3MAS mutant: K65R, K160R and K490R; mutated SIM
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208039-plasmid-map-sequence-id-410207

    Enlarge

Showing: 511 - 540 of 1225 results