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Addgene

We narrowed to 4,162 results for: 1-Oct

Showing: 341 - 360 of 4162 results
  1. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid
    #70655
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pGADT7-AP-1 σ1C

    Plasmid
    #198336
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    AP-1 σ1C
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    S148C substitution
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. GST-PC-1-CTS

    Plasmid
    #86067
    Purpose
    A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Insert
    Polycystin-1 (PKD1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains the region from 4297-4303
    Promoter
    tac
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid
    #70651
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. Cer(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #55707
    Purpose
    An amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.
    Depositor
    Insert
    mCer(1-158)-beta 1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pGADT7-AP-1 σ1B

    Plasmid
    #198335
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    AP-1 σ1B
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pGADT7-AP-1 σ1A

    Plasmid
    #198334
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    AP-1 σ1A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA-Prom1 D-1

    Plasmid
    #184013
    Purpose
    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 685-706.
    Depositor
    Insert
    Prom1 SV8(-Ex19a) D-1 (Prom1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 685-706.
    Promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 341 - 360 of 4162 results