Skip to main content
Addgene
Showing: 341 - 360 of 4219 results

  1. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis, Strep II, and mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

    Enlarge

  2. pExp-DsbA(SS)

    Plasmid
    #112563
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(SS) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112563-plasmid-map-sequence-id-217221

    Enlarge

  3. pExp-Bla

    Plasmid
    #112561
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-Bla and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112561-plasmid-map-sequence-id-217226

    Enlarge

  4. pExp-IF2

    Plasmid
    #112567
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-IF2 and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112567-plasmid-map-sequence-id-217215

    Enlarge

  5. pExp-AFV

    Plasmid
    #112560
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and AFV (Acidianus Filamentous Virus 1 protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-AFV and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112560-plasmid-map-sequence-id-217216

    Enlarge

  6. PS-CFP2-Zyxin-6

    Plasmid
    #57256
    Purpose
    Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    Zyxin (ZYX Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    S11P, G52R, E62D
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57256-plasmid-map-sequence-id-210653

    Enlarge

  7. AAV-CAG-jGCaMP8s-WPRE

    Plasmid
    #179256
    Purpose
    AAV-mediated expression of GCaMP8s under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179256-plasmid-map-sequence-id-354036

    Enlarge

  9. pExp-DsbA

    Plasmid
    #112562
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-DsbA(AA20-208) and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112562-plasmid-map-sequence-id-217223

    Enlarge

  10. pAc5.1B-EGFP-DmeIF5B_Q

    Plasmid
    #147256
    Purpose
    Insect Expression of DmeIF5B
    Depositor
    Elisa Izaurralde
    Insert
    DmeIF5B (eIF5B Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    two non silent mutations A441D and I857T compared…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147256-plasmid-map-sequence-id-289703

    Enlarge

  11. 5UBA (RPUSD4)

    Plasmid
    #101256
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RPUSD4 (RPUSD4 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101256-plasmid-map-sequence-id-197526

    Enlarge

  12. PARP9

    Plasmid
    #73256
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP9 (PARP9 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73256-plasmid-map-sequence-id-136235

    Enlarge

  16. pGWB14-LYX038

    Plasmid
    #126256
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf01710g04007.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    126256-plasmid-map-sequence-id-246488

    Enlarge

Showing: 341 - 360 of 4219 results